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Titolo:
CORTICOSTEROID RECEPTOR MESSENGER-RNA EXPRESSION IS UNAFFECTED BY CORTICOSTEROIDS IN RAT-KIDNEY, HEART, AND COLON
Autore:
ESCOUBET B; COUREAU C; BLOTCHABAUD M; BONVALET JP; FARMAN N;
Indirizzi:
UNIV PARIS 07,FAC MED XAVIER BICHAT,INSERM U426,INST FEDERAT RECH CELLULES EPITHELIALES,BP 416 F-75870 PARIS 18 FRANCE UNIV PARIS 07,FAC MED XAVIER BICHAT,INSERM U246,INST FEDERAT RECH CELLULES EPITHELIALES F-75870 PARIS 18 FRANCE
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 5, volume: 39, anno: 1996,
pagine: 1343 - 1353
SICI:
0363-6143(1996)39:5<1343:CRMEIU>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MINERALOCORTICOID RECEPTOR; MESSENGER-RNA; DEXAMETHASONE BINDING; RIBONUCLEIC-ACID; RABBIT KIDNEY; ALDOSTERONE; NEPHRON; LOCALIZATION; MODULATION; IMMUNOLOCALIZATION;
Keywords:
ALDOSTERONE; DEXAMETHASONE; RIBONUCLEASE PROTECTION; MINERALOCORTICOID RECEPTOR; GLUCOCORTICOID RECEPTOR; MESSENGER RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
B. Escoubet et al., "CORTICOSTEROID RECEPTOR MESSENGER-RNA EXPRESSION IS UNAFFECTED BY CORTICOSTEROIDS IN RAT-KIDNEY, HEART, AND COLON", American journal of physiology. Cell physiology, 39(5), 1996, pp. 1343-1353

Abstract

Hormones can regulate the expression of their own receptor. We have examined whether adrenalectomy (ADX) and hormone replacement by physiological doses of aldosterone or dexamethasone could modulate the expression of glucocorticoid receptor (GR) or mineralocorticoid receptor (MR) at the mRNA level in the rat kidney, distal colon, and heart. Adult rats were adrenalectomized and received or did not receive an infusionof aldosterone (5 mu g . 100 g(-1). day(-1)) or dexamethasone (10 mu g . 100 g(-1). day(-1)). No significant change in steady-state levels of both MR and GR mRNA was detectable by using ribonuclease (RNase) protection assay (RPA) after either ADX or hormone replacement. Because the kidney is heterogeneous with regard to MR expression, RPA was adapted for measurements on microdissected nephron segments. GR mRNA is expressed at comparable levels all along the nephron, whereas MR mRNA isrestricted to the distal nephron. No effect of ADX on GR and MR mRNA levels was detected in any nephron segment that was either aldosteronesensitive or insensitive. In situ hybridization confirmed the absenceof corticosteroid-dependent modulation of MR mRNA in all kidney cell types. We conclude that variations of corticosteroid status do not affect MR and GR mRNA steady-state levels in heart, colon, and kidney andthus do not participate to the functional adaptations that are known to depend on hormonal status.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:30:13