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Titolo:
MUTATOR TRANSFER-RNAS ARE ENCODED BY THE ESCHERICHIA-COLI MUTATOR GENES MUTA AND MUTC - A NOVEL PATHWAY FOR MUTAGENESIS
Autore:
SLUPSKA MM; BAIKALOV C; LLOYD R; MILLER JH;
Indirizzi:
UNIV CALIF LOS ANGELES,DEPT MICROBIOL & MOLEC GENET LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,INST MOLEC BIOL LOS ANGELES CA 90024
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 9, volume: 93, anno: 1996,
pagine: 4380 - 4385
SICI:
0027-8424(1996)93:9<4380:MTAEBT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-III HOLOENZYME; MISMATCH REPAIR; TRANSFER-RNA; HUMAN-FIBROBLASTS; EPSILON-SUBUNIT; DNA-REPLICATION; ERROR THEORY; MUTATIONS; MECHANISMS; HOMOLOG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Slupska et al., "MUTATOR TRANSFER-RNAS ARE ENCODED BY THE ESCHERICHIA-COLI MUTATOR GENES MUTA AND MUTC - A NOVEL PATHWAY FOR MUTAGENESIS", Proceedings of the National Academy of Sciences of the United Statesof America, 93(9), 1996, pp. 4380-4385

Abstract

We have previously described the mutator alleles mutA and mutC, whichmap at 95 minutes acid 42 minutes, respectively, on the Escherichia coli genetic map and which stimulate transversions; the A . T --> T . Aand G . C --> T . A substitutions are the most prominent. In this study we show that both mutA and mutC result from changes in the anticodon In one of four copies of the same glycine tRNA, at either the glyV or the glyW locus. This change results in a tRNA that inserts glycine at aspartic acid codons. In view of previous studies of missense suppressor tRNAs, the mistranslation of aspartic acid codons is assumed to occur at approximate to 1-2%. We postulate that the mutator tRNA effectis exerted by generating a mutator polymerase and suggest that the epsilon subunit of DNA polymerase, which pro,ides a proofreading function, is the most likely target. The implications of these findings for the contribution of mistranslation to observed spontaneous mutation rates in wild-type strains, as well as other cellular phenomena such as aging, are discussed.

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Documento generato il 29/11/20 alle ore 06:37:18