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Titolo:
MAMMALIAN PHOSPHOLIPASE-D - PHOSPHATIDYLETHANOLAMINE AS AN ESSENTIAL COMPONENT
Autore:
NAKAMURA SI; KIYOHARA Y; JINNAI H; HITOMI T; OGINO C; YOSHIDA K; NISHIZUKA Y;
Indirizzi:
KOBE UNIV,SCH MED,DEPT BIOCHEM KOBE HYOGO 650 JAPAN KOBE UNIV,BIOSIGNAL RES CTR KOBE HYOGO 657 JAPAN
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 9, volume: 93, anno: 1996,
pagine: 4300 - 4304
SICI:
0027-8424(1996)93:9<4300:MP-PAA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDENTIFICATION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
S.I. Nakamura et al., "MAMMALIAN PHOSPHOLIPASE-D - PHOSPHATIDYLETHANOLAMINE AS AN ESSENTIAL COMPONENT", Proceedings of the National Academy of Sciences of the United Statesof America, 93(9), 1996, pp. 4300-4304

Abstract

Bovine kidney phospholipase D (PLD) was assayed by measuring the formation of phosphatidylethanol from added radioactive phosphatidylcholine (PtdCho) in the presence of ethanol, guanosine 5'-[gamma-thio]triphosphate, ammonium sulfate, and cytosol factor that contained small GTP-binding regulatory proteins. The PLD enzyme associated with particulate fractions was solubilized by deoxycholate and partially purified by chromatography on a heparin-Sepharose column. This PLD preferentially used PtdCho as substrate. After purification, the enzyme per se showedlittle or practically no activity but required an additional factor for the enzymatic reaction. This factor was extracted with chloroform/methanol directly from particulate fractions of various tissues, including kidney, liver, and brain, and identified as phosphatidylethanolamine (PtdEtn), although this phospholipid did not serve as a good substrate. Plasmalogen-rich PtdEtn, dioleoyl-PtdEtn, and L-alpha-palmitoyl-beta-linoleoyl-PtdEtn were effective, but dipalmitoyl-PtdEtn was inert. Sphingomyelin was 30% as active as PtdEtn. The results suggest that mammalian PLD reacts nearly selectively with PtdCho in the form of mixed micelles or membranes with other phospholipids, especially PtdEtn.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 04:53:51