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Titolo:
IN-VIVO INDUCTION OF CYTOCHROME-P450 CYP3A EXPRESSION IN RAT LEUKOCYTES USING VARIOUS INDUCERS
Autore:
MAHNKE A; ROOS PH; HANSTEIN WG; CHABOT GG;
Indirizzi:
INST GUSTAVE ROUSSY,CNRS URA 147,PHARMACOTOXICOL & PHARMACOGENET LAB,RES PAVILL 2 F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,CNRS URA 147,PHARMACOTOXICOL & PHARMACOGENET LAB F-94805 VILLEJUIF FRANCE RUHR UNIV BOCHUM,INST PHYSIOL CHEM D-44780 BOCHUM GERMANY
Titolo Testata:
Biochemical pharmacology
fascicolo: 11, volume: 51, anno: 1996,
pagine: 1579 - 1582
SICI:
0006-2952(1996)51:11<1579:IIOCCE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MESSENGER-RNA; ENZYMES; ACID; HYDROXYLASE; LYMPHOCYTES; CLONING; TISSUES; P-450;
Keywords:
CYTOCHROMES P450; CYP3A; INDUCTION; LEUKOCYTE; NONINVASIVE TEST; WESTERN BLOT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A. Mahnke et al., "IN-VIVO INDUCTION OF CYTOCHROME-P450 CYP3A EXPRESSION IN RAT LEUKOCYTES USING VARIOUS INDUCERS", Biochemical pharmacology, 51(11), 1996, pp. 1579-1582

Abstract

Although the induction of cytochromes P450 3A (CYP3A) is relatively well characterized in liver, its inducibility in an easily available tissue such as the peripheral leukocytes is not known. The purpose of this study was, therefore, to determine if CYP3A is inducible in vivo inperipheral leukocytes. Microsomes from rat leukocytes and liver were examined for CYP3A protein expression using Western blotting with a rabbit polyclonal antibody against rat CYP3A. Although CYP3A was not detected in control leukocytes, in vivo treatment with known CYP3A inducers (dexamethasone, clotrimazole, phenobarbital, pregnenolone-16 alpha-carbonitrile) resulted in CYP3A leukocyte levels of 0.2-0.8 pmol/mg protein. This leukocyte induction was approximately 1000-fold lower thanin induced liver. Interestingly, there was an apparent linear relationship between leukocyte and liver CYP3A contents (r(2) = 0.748, n = 29). These results not only demonstrate for the first time that CYP3A isinducible in rat leukocytes after in vivo treatment with various CYP3A inducers, but also suggest that peripheral leukocytes could be used to assess induction in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:58:35