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Titolo:
INTRACELLULAR GLUTATHIONE IN THE PERIPHERAL-BLOOD CELLS OF HIV-INFECTED PATIENTS - FAILURE TO SHOW A DEFICIENCY
Autore:
PIRMOHAMED M; WILLIAMS D; TINGLE MD; BARRY M; KHOO SH; OMAHONY C; WILKINS EGL; BRECKENRIDGE AM; PARK BK;
Indirizzi:
UNIV LIVERPOOL,DEPT PHARMACOL & THERAPEUT,POB 147 LIVERPOOL L69 3BX MERSEYSIDE ENGLAND UNIV LIVERPOOL,DEPT PHARMACOL & THERAPEUT LIVERPOOL L69 3BX MERSEYSIDE ENGLAND CHESTER ROYAL INFIRM,DEPT GENITOURINARY MED CHESTER CHESHIRE ENGLAND N MANCHESTER GRP HOSP,INFECT DIS UNIT MANCHESTER LANCS ENGLAND
Titolo Testata:
AIDS
fascicolo: 5, volume: 10, anno: 1996,
pagine: 501 - 507
SICI:
0269-9370(1996)10:5<501:IGITPC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; N-ACETYLCYSTEINE; METABOLISM; CYSTEINE; DAPSONE; MONOBROMOBIMANE; HYDROXYLAMINE; INDIVIDUALS; DISEASE; PLASMA;
Keywords:
HIV; AIDS; GLUTATHIONE; DRUG HYPERSENSITIVITY; TRIMETHOPRIM-SULFAMETHOXAZOLE; METHEMOGLOBINEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M. Pirmohamed et al., "INTRACELLULAR GLUTATHIONE IN THE PERIPHERAL-BLOOD CELLS OF HIV-INFECTED PATIENTS - FAILURE TO SHOW A DEFICIENCY", AIDS, 10(5), 1996, pp. 501-507

Abstract

Objective: To determine whether HIV-infected patients have a deficiency of intracellular glutathione (GSH) in peripheral blood mononuclear cells (PBMC) and erythrocytes. Design: initial experiments determiningthe stability of intracellular GSH preceded the measurement of GSH levels in 33 HIV-positive patients and 40 control subjects within 1 h ofisolation of their blood cells. in addition, the susceptibility of erythrocytes to dapsone hydroxylamine-induced methaemoglobinaemia was evaluated. Methods: GSH levels were determined by an high-performance liquid chromatography method utilizing a fluorescent probe, monobromobimane. The bimane-GSH adduct formed in PBMC was also characterized by mass spectrometry. Methaemoglobin formation on exposure to dapsone hydroxylamine was determined spectrophotometrically. Results: GSH levels remained stable for only 1 h after cell isolation, thereafter showing a decrease of 20 and 60% at 4 and 24 h, respectively. There was no difference in the CSH levels in PBMC and erythrocytes of the HIV-positive patients compared with controls. The GSH levels were not related to thedisease stage or to CD4+ cell counts. There was no difference in GSH levels in PBMC taken from trimethoprimsulphamethoxazole-hypersensitiveand non-hypersensitive patients. Methaemoglobinaemia on exposure of erythrocytes to dapsone hydroxylamine was concentration-dependent, but there was no significant difference between patients and controls. Conclusion: In contrast to previous studies, no deficiency of intracellular GSH in the PBMC and erythrocytes of HIV-infected patients was found. The discrepancy between studies may be methodological reflecting theinstability of GSH, which requires prompt sample analysis.

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Documento generato il 02/07/20 alle ore 22:41:28