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Titolo:
BIOLOGICAL FUNCTION AND DISTRIBUTION OF HUMAN INTERLEUKIN-12 RECEPTOR-BETA CHAIN
Autore:
WU CY; WARRIER RR; CARVAJAL DM; CHUA AO; MINETTI LJ; CHIZZONITE R; MONGINI PKA; STERN AS; GUBLER U; PRESKY DH; GATELY MK;
Indirizzi:
HOFFMANN LA ROCHE INC,DEPT INFLAMMAT AUTOIMMUNE DIS NUTLEY NJ 07110 HOFFMANN LA ROCHE INC,DEPT INFLAMMAT AUTOIMMUNE DIS NUTLEY NJ 07110 HOSP JOINT DIS & MED CTR,DEPT RHEUMATOL & MOLEC MED NEW YORK NY 00000
Titolo Testata:
European Journal of Immunology
fascicolo: 2, volume: 26, anno: 1996,
pagine: 345 - 350
SICI:
0014-2980(1996)26:2<345:BFADOH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED HUMAN LYMPHOBLASTS; MONOCLONAL-ANTIBODIES; IL-12 RECEPTOR;
Keywords:
INTERLEUKIN-12; INTERLEUKIN-12 RECEPTOR; INTERLEUKIN-12 RECEPTOR BETA CHAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
C.Y. Wu et al., "BIOLOGICAL FUNCTION AND DISTRIBUTION OF HUMAN INTERLEUKIN-12 RECEPTOR-BETA CHAIN", European Journal of Immunology, 26(2), 1996, pp. 345-350

Abstract

We previously described the cloning of a cDNA encoding an interleukin-12 receptor (IL-12R) subunit. designated beta, that bound IL-12 with low affinity when expressed in COS cells. We now report that a pair ofmonoclonal antibodies (mAb), 2B10 and 2.4E6, directed against different epitopes on the IL-12R beta chain. when used in combination, strongly inhibited IL-12 induced proliferation of activated T cells. IL-12-induced secretion of interferon-gamma by resting peripheral blood mononuclear cells (PBMC). and IL-12-mediated lymphokine-activated killer cell activation. The mAb had no effect on lymphoblast proliferation induced by IL-2, -4, or -7. Thus, the IL-12R on both T and natural killer (NK) cells. We previously observed that high affinity IL-12R were expressed on activated T and NK cells. but not B cells. Studies using flowcytometry and reverse transcription-polymerase chain reaction analysis showed that IL-12R beta chain was expressed on several human T. NK. and (surprisingly) B cell lines. but not on non-lymphohematopoietic cell lines. The Kit225/K6 (T cell) and SKW6.4 (B cell) lines were found to express the greatest amounts of IL-12R beta chain (800-2500 sites/cell): however Kit225/K6 but not SKW6.4 cells bound IL-12. Similar to SK6.4 B cells. activated tonsillar B lymphocytes expressed IL-12 binding. Likewise, up to 72% of resting PBMC from normal volunteer donors expressed IL-12R beta, but did not bind measurable amounts of IL-12. These results indicate that expression of IL-12R. We speculate that expression of functional. high-affinity IL-12R may require the presence of a second subunit that is more restricted in its expression than IL-12Rbeta.

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Documento generato il 21/09/20 alle ore 02:36:51