Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE P53 SIGNAL-TRANSDUCTION PATHWAY IS INTACT IN HUMAN NEUROBLASTOMA DESPITE CYTOPLASMIC LOCALIZATION
Autore:
GOLDMAN SC; CHEN CY; LANSING TJ; GILMER TM; KASTAN MB;
Indirizzi:
JOHNS HOPKINS UNIV HOSP,720 RUTLAND AVE,345 ROSS BLDG BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,JOHNS HOPKINS ONCOL CTR BALTIMORE MD 21205 GLAXO WELLCOME RES INST RES TRIANGLE PK NC 00000
Titolo Testata:
The American journal of pathology
fascicolo: 5, volume: 148, anno: 1996,
pagine: 1381 - 1385
SICI:
0002-9440(1996)148:5<1381:TPSPII>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUTATIONS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
S.C. Goldman et al., "THE P53 SIGNAL-TRANSDUCTION PATHWAY IS INTACT IN HUMAN NEUROBLASTOMA DESPITE CYTOPLASMIC LOCALIZATION", The American journal of pathology, 148(5), 1996, pp. 1381-1385

Abstract

Mutations of the p53 tumor suppressor gene are rarely found in neuroblastoma. Though typically a nuclear protein, a number of tumor cell types have recently been reported to exhibit cytoplasmic p53 immunostaining, and it has been suggested that altered cellular localization is another mechanism of inhibiting p53 function. We examined p53 protein expression, localization, and function in neuroblastoma cell lines withwild-type p53 genes. Basal p53 levels were largely confined to the cytoplasmic compartment in these cells. However, after irradiation, p53 protein levels increased predominately in the nucleus. Transcriptionalactivity of p53 was intact in these cells because ''downstream'' proteins, p21(WAF1) and MDM2, were induced by irradiation. In contrast to a neuroblastoma cell line harboring a mutant p53 gene, the neuroblastoma cells with wild-type protein were associated with an intact G1 arrest after DNA damage. The induced also appeared functional as measured by its capacity to bind to a DNA oligomer containing a p53-consensus sequence. We have concluded that although p53 expression in neuroblastoma cells is primarily localized to the cytosol, ionizing radiation induces a functional p53 protein in the nucleus and that this cytoplasmicsequestration of p53 in human neuroblastoma is not a mechanism of inactivating p53 function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 16:01:33