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Titolo:
SIMULTANEOUS DETERMINATION OF DEXTROMETHORPHAN AND DEXTRORPHAN IN HUMAN PLASMA, URINE AND CEREBROSPINAL-FLUID BY HPLC WITH FLUORESCENCE DETECTION
Autore:
KIMISKIDIS VK; KAZIS AD; NIOPAS I;
Indirizzi:
ARISTOTELIAN UNIV THESSALONIKI,G PAPANICOLAOU HOSP,DEPT NEUROL,SCH MED GR-54006 THESSALONIKI GREECE ARISTOTELIAN UNIV THESSALONIKI,DEPT PHARM GR-54006 THESSALONIKI GREECE
Titolo Testata:
Journal of liquid chromatography & related technologies
fascicolo: 8, volume: 19, anno: 1996,
pagine: 1267 - 1275
SICI:
1082-6076(1996)19:8<1267:SDODAD>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; METABOLITES; HYDROXYLATION; POLYMORPHISMS; DEBRISOQUIN; OXIDATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
V.K. Kimiskidis et al., "SIMULTANEOUS DETERMINATION OF DEXTROMETHORPHAN AND DEXTRORPHAN IN HUMAN PLASMA, URINE AND CEREBROSPINAL-FLUID BY HPLC WITH FLUORESCENCE DETECTION", Journal of liquid chromatography & related technologies, 19(8), 1996, pp. 1267-1275

Abstract

Dextromethorphan is a non-narcotic antitussive drug that recently attracted interest because of its anticonvulsant and neuroprotective properties. Dextromethorphan undergoes polymorphic oxidation and 5-10% of human subjects are characterised as poor metabolizers on the basis of the formation rate of its O-demethylated metabolite, dextrorphan catalysed by isozyme CYP2D6. A sensitive, selective and reliable high performance liquid chromatographic (HPLC) method for the simultaneous determination of dextromethorphan and dextrorphan in human plasma, urine, and cerebrospinal fluid, is described, using levallorphan as the internal standard. The analytes were extracted from biological fluids by a liquid-liquid extraction procedure. The chromatographic separation was performed on a 5-mu M cyano analytical column (220 x 4.6 mm) using a mixture of acetonitrile/distilled water/n-octylamine (19/80.95/0.05, v/v) pH 2.8 as the mobile phase with a flow rate of 1.0 mL/min at 40 degrees C and the chromatographic peaks were measured by fluorescence detection. The calibration curves were linear with a correlation coefficient of 0.999 or better from 1-100, 5-500, and 1-100 ng/mL for both drugs in plasma, urine, and cerebrospinal fluid, respectively The method was not interfered with by other endogenous components or concurrent antiepileptic drugs. The within-day precision of the method was evaluated for three concentrations and was found to be less than 6%, and the accuracy was nearly 100%.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:27:22