Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
METHYLATION PATTERN OF ACTIVATOR TRANSPOSASE BINDING-SITES IN MAIZE ENDOSPERM
Autore:
WANG LH; HEINLEIN M; KUNZE R;
Indirizzi:
UNIV MUNICH,INST GENET & MIKROBIOL,MARIA WARD STR 1A D-80638 MUNICH GERMANY UNIV MUNICH,INST GENET & MIKROBIOL D-80638 MUNICH GERMANY
Titolo Testata:
The Plant cell
fascicolo: 4, volume: 8, anno: 1996,
pagine: 747 - 758
SICI:
1040-4651(1996)8:4<747:MPOATB>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZEA-MAYS-L; TRANSGENIC TOBACCO PLANTS; ELEMENT-AC; SUBTERMINAL SEQUENCES; PUTATIVE TRANSPOSASE; MOLECULAR ANALYSIS; DNA METHYLATION; SYNTHASE GENE; DS; LOCUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
L.H. Wang et al., "METHYLATION PATTERN OF ACTIVATOR TRANSPOSASE BINDING-SITES IN MAIZE ENDOSPERM", The Plant cell, 8(4), 1996, pp. 747-758

Abstract

The maize transposable element Activator (Ac) transposes after replication from only one of the two daughter chromatids. It has been suggested that DNA methylation in conjunction with methylation-sensitive transposase binding to DNA may control the association of Ac transposition and replication. We present hem a detailed genomic sequencing analysis of the cytosine methylation patterns of the transposase binding sites within both Ac ends in the wx-m9::Ac allele, where Ac is inserted into the tenth exon of the Waxy gene. The Ac elements in wx-m9::Ac kernels exhibit intriguing methylation patterns and fall into two distinctgroups. Approximately 50% of the elements are fully unmethylated at cytosine residues through the 256 nucleotides at the 5' end (the promoter end). The other half is partially methylated between Ac residues 27and 92. In contrast, at the 3' end, all Ac molecules are heavily methylated between residues 4372 and 4554. The more internally located Ac sequences and the flanking Waxy DNA are unmethylated. Although most methylated cytosines in Ac are in the symmetrical CpG and CpNpG arrangements, nonsymmetrical cytosine methylation is also common in the hypermethylated regions of Ac. These results suggest a model in which differential activation of transposon ends by hemimethylation controls the chromatid selectivity of transposition and the association with replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:07:25