Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EXPRESSION OF FUNCTIONAL MELANOCORTIN-1 RECEPTORS IN INSECT CELLS
Autore:
SCHIOTH HB; KUUSINEN A; MUCENIECE R; SZARDENINGS M; KEINANEN K; WIKBERG JES;
Indirizzi:
UPPSALA UNIV,DEPT PHARMACEUT PHARMACOL UPPSALA SWEDEN VTT,BIOTECHNOL & FOOD RES ESPOO FINLAND INST ORGAN SYNTH,PHARMACOL LAB RIGA LATVIA WA PHARM UMEA SWEDEN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 221, anno: 1996,
pagine: 807 - 814
SICI:
0006-291X(1996)221:3<807:EOFMRI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOCYTE-STIMULATING HORMONE; MOLECULAR-CLONING; IDENTIFICATION; PURIFICATION; SYSTEM; GENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
H.B. Schioth et al., "EXPRESSION OF FUNCTIONAL MELANOCORTIN-1 RECEPTORS IN INSECT CELLS", Biochemical and biophysical research communications, 221(3), 1996, pp. 807-814

Abstract

We expressed epitope-tag,oed human melanocortin 1 receptor (MC1R) in insect cells using two different recombinant baculovirus constructs; one of which encoded MC1R with an N-terminal Flag epitope and a C-terminal polyHis tag, while the other encoded the MC1R with a C-terminal Myc tag. The constructs were used to infect Sf9 insect cells. For both constructs, immunoblotting with tag-specific antibodies demonstrated the presence of the receptor in the infected cells. The infected Sf9 cells were characterized by radioligand binding using [I-125][Nle(4), D-Phe(7)]alpha-MSH. Both saturation and competition analysis, using alpha-, beta-, and gamma(1)-MSH on the tagged MC1R expressed in the insect cells, gave binding constants and potency orders that were undistinguishable from those obtained on MC1R expressed in COS cells. The expression level obtained (in the order of pmoles of binding sites per mg of protein) will now facilitate attempts to purify the receptor. This is the first report that demonstrates a functional expression of recombinant melanocortin receptor in nonmammalian cells. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:31:12