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Titolo:
AUTOANTIBODIES TO THE COLLAGENOUS REGION OF C1Q OCCUR IN 3 STRAINS OFLUPUS-PRONE MICE
Autore:
HOGARTH MB; NORSWORTHY PJ; ALLEN PJ; TRINDER PKE; LOOS M; MORLEY BJ; WALPORT MJ; DAVIES KA;
Indirizzi:
HAMMERSMITH HOSP,RHEUMATOL UNIT,RPMS,DU CANE RD LONDON W12 0NN ENGLAND HAMMERSMITH HOSP,RHEUMATOL UNIT,RPMS LONDON W12 0NN ENGLAND UNIV MAINZ,INST MED MICROBIOL & HYG MAINZ GERMANY
Titolo Testata:
Clinical and experimental immunology
fascicolo: 2, volume: 104, anno: 1996,
pagine: 241 - 246
SICI:
0009-9104(1996)104:2<241:ATTCRO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE C1Q; MOUSE C1Q; B-CHAIN; ANTIBODIES; IGG; ERYTHEMATOSUS; CLQ; NEPHRITIS; VASCULITIS; COMPLEMENT;
Keywords:
ANTI-C1Q ANTIBODIES; MRL/LPR; BXSB; NZB/W; LUPUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M.B. Hogarth et al., "AUTOANTIBODIES TO THE COLLAGENOUS REGION OF C1Q OCCUR IN 3 STRAINS OFLUPUS-PRONE MICE", Clinical and experimental immunology, 104(2), 1996, pp. 241-246

Abstract

We have developed an ELISA to measure murine autoantibodies to the collagenous region (CLR) of Clq, using the whole human Clq molecule as the solid-phase ligand, in the presence of 1 M NaCl. The assay was validated by testing positive sera from 20 mice using purified mouse Clq, and from 10 mice using purified human Clq-CLR, as the solid-phase ligands. There were highly significant correlations between results obtained with human Clq(whole molecule) and: (i) mouse Clq (r(Sp) = 0.73, P < 0.001), and (ii) human Clq-CLR alone (r(Sp) = 0.86, P = 0.001). Antibodies to Clq were measured in 53 MRL/lpr, 17 BXSB and 25 NZB/W lupus-prone mice. Median (range) anti-Clq (CLR) antibody levels in MRL/lpr, BXSB, and NZB/W autoimmune mice aged 3 months were 22 (16-66), 21 (17-39) and 19 (15-27) EU, respectively. The median anti-Clq antibody level in MRL/lpr mice aged 5 months was 76 (35-142) EU, significantly higher than that at 3 months (U = 558, P < 0.0005). Median anti-Clq antibody level in NZB/W mice at 8 months was 37 (13-74) EU and in BXSB mice at 11 months was 62 (31-231) EU, significantly higher than corresponding values at 3 months (U = 326, and U = 4, P < 0.001, respectively). This is the first demonstration of anti-Clq (CLR) antibodies in NZB/W and BXSB mice. The pathologic significance and the potential utility ofthese antibodies for monitoring disease in lupus-prone mice are underevaluation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:02:02