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Titolo:
DIFFERENTIAL EXPRESSION OF LAMININ CHAIN-SPECIFIC MESSENGER-RNA TRANSCRIPTS DURING MOUSE PREIMPLANTATION EMBRYO DEVELOPMENT
Autore:
SHIM C; KWON HB; KIM K;
Indirizzi:
SEOUL NATL UNIV,COLL NAT SCI,DEPT BIOL MOLEC SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,COLL NAT SCI,DEPT BIOL MOLEC SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,SRC CELL DIFFERENTIAT SEOUL SOUTH KOREA CHUNNAM NATL UNIV,DEPT BIOL KWANGJU SOUTH KOREA CHUNNAM NATL UNIV,HORMONE RES CTR KWANGJU SOUTH KOREA
Titolo Testata:
Molecular reproduction and development
fascicolo: 1, volume: 44, anno: 1996,
pagine: 44 - 55
SICI:
1040-452X(1996)44:1<44:DEOLCM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; ACTIN MESSENGER-RNA; INNER CELL MASS; EXTRACELLULAR-MATRIX; NUCLEOTIDE-SEQUENCE; MULTIDOMAIN PROTEIN; DROSOPHILA LAMININ; BASEMENT-MEMBRANES; GENE-EXPRESSION; A-CHAIN;
Keywords:
LAMININ; RT-PCR; PREIMPLANTATION EMBRYO; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
C. Shim et al., "DIFFERENTIAL EXPRESSION OF LAMININ CHAIN-SPECIFIC MESSENGER-RNA TRANSCRIPTS DURING MOUSE PREIMPLANTATION EMBRYO DEVELOPMENT", Molecular reproduction and development, 44(1), 1996, pp. 44-55

Abstract

Laminin is the first extracellular matrix protein that has been shownto be synthesized in preimplantation mouse embryos. In the present study, chain-specific expression patterns of laminin mRNAs were examinedby semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). During preimplantation mouse embryo development, temporal expression patterns of laminin chain mRNAs were somewhat differential: B1chain mRNA was first detectable at the late two-cell stage and its level was gradually increased by the blastocyst stage. In contrast, B2 and A chain mRNAs first appeared at the morula and blastocyst stages, respectively. At the blastocyst stage, all of the laminin chain mRNAs were highly detected compared to the earlier stages. When embryos were flushed at the morula stage and cultured in vitro, all laminin chain mRNA levels were decreased or not changed in the process of blastocoeleexpansion. In contrast, in the in vivo condition where embryos at different stages of blastocyst were flushed at different time points, laminin chain mRNA levels were increased as a function of blastocoele expansion. These changes in laminin mRNAs were parallel with its receptors such as integrin alpha 3 and alpha 6. 3-lsobutyl-1-methylxanthine (IBMX), which is known to be a potent activator of blastocoele expansionand regulates cAMP metabolism, upregulated laminin expression (exceptB1 chain) in blastocysts cultured in vitro. In vitro cultured embryosnormally developed up to the late blastocyst, although their development was delayed compared with the in vivo condition where laminin geneexpression was gradually increased as the blastocoele expanded. Theseresults indicate that laminin expression may not be involved directlyin the regulation of blastocoele expansion. The uterine environment enclosing the preimplantation embryos appears, therefore, to play an important role in the regulation of laminin gene expression during blastocyst development. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:10:48