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Titolo:
CLONING, EXPRESSION, AND LOCALIZATION OF A NEW MEMBER OF A PARACENTROTUS-LIVIDUS CELL-SURFACE MULTIGENE FAMILY
Autore:
MONTANA G; ROMANCINO DP; DICARLO M;
Indirizzi:
CNR,IST BIOL SVILUPPO,VIA ARCHIRAFI 20 I-90123 PALERMO ITALY CNR,IST BIOL SVILUPPO I-90123 PALERMO ITALY
Titolo Testata:
Molecular reproduction and development
fascicolo: 1, volume: 44, anno: 1996,
pagine: 36 - 43
SICI:
1040-452X(1996)44:1<36:CEALOA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EMBRYO; 5' UNTRANSLATED REGION; MATERNAL MESSENGER-RNA; XENOPUS OOCYTES; PROTEIN; GENE; SEQUENCE; TRANSLOCATION; GASTRULATION; LAEVIS;
Keywords:
SEA URCHIN EMBRYO; CELL SURFACE PROTEIN; IN SITU HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
G. Montana et al., "CLONING, EXPRESSION, AND LOCALIZATION OF A NEW MEMBER OF A PARACENTROTUS-LIVIDUS CELL-SURFACE MULTIGENE FAMILY", Molecular reproduction and development, 44(1), 1996, pp. 36-43

Abstract

We have isolated and characterized a cDNA clone corresponding to a new member of bep (butanol, extracted, proteins) Paracentrotus lividus multigene family coding for cell surface proteins. The cDNA, called bep3, encodes a 370 amino acid protein and shares the same structural organization in the coding region with other members of the same gene family already characterized. Expression of this clone studied by Northern blot and by whole mount hybridization shows that the bep3 messenger is transcribed during oogenesis and utilized till the gastrula stage, whereas at the prism stage, unlike other members of the same gene family, new synthesis of messenger occurs. By whole mount hybridization spatial distribution of bep3 messenger in egg and embryos is established. This messenger appears located in the animal half of the unfertilized egg and moves to the cortical zone after fertilization; it is not present in the structures derived by the vegetal part of the embryo, such as the micromeres of the 16-cell stage, the primary mesenchyme cellsof the blastula, and the primary intestine of the gastrula. At the prism stage instead, hybridization of bep3 messenger is restricted to the part of the embryo that will give origin to the oral region as successively confirmed by hybridization at the pluteus stage. The result ofwhole mount hybridization was confirmed by Northern blot hybridization of separated meso-macromere and micromere RNAs. A Southern blot experiment demonstrates that bep3 is codified by a single copy gene. Conservation of the bep multigene family in several Mediterranean and Japanese sea urchin species has also been analyzed. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 06:43:56