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Titolo:
EFFECT OF SPECIMEN COLLECTION TECHNIQUES, TRANSPORT MEDIA, AND INCUBATION OF CULTURES ON THE DETECTION RATE OF HELICOBACTER-PYLORI
Autore:
VANDERHULST RWM; VERHEUL SB; WEEL JFL; GERRITS Y; TENKATE FJW; DANKERT J; TYTGAT GNJ;
Indirizzi:
ACAD MED CTR,DEPT GASTROENTEROL,MEIBERGDREEF 9 1105 AZ AMSTERDAM NETHERLANDS ACAD MED CTR,DEPT MED MICROBIOL 1105 AZ AMSTERDAM NETHERLANDS ACAD MED CTR,DEPT HISTOPATHOL 1105 AZ AMSTERDAM NETHERLANDS
Titolo Testata:
European journal of clinical microbiology & infectious diseases
fascicolo: 3, volume: 15, anno: 1996,
pagine: 211 - 215
SICI:
0934-9723(1996)15:3<211:EOSCTT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAMPYLOBACTER-PYLORI; BIOPSY SPECIMENS; RAPID DIAGNOSIS; BRUSH CYTOLOGY; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
R.W.M. Vanderhulst et al., "EFFECT OF SPECIMEN COLLECTION TECHNIQUES, TRANSPORT MEDIA, AND INCUBATION OF CULTURES ON THE DETECTION RATE OF HELICOBACTER-PYLORI", European journal of clinical microbiology & infectious diseases, 15(3), 1996, pp. 211-215

Abstract

Culture and histologic examination are considered ''gold standard'' methods for the detection of Helicobacter pylori, but discrepancies mayoccur with either method. Failure to detect Helicobacter pylori may be due to sampling error, inappropriate transport or culture media, or insufficient duration of the incubation period, Rates of detection of Helicobacter pylori by culture and histopathologic examination of gastric mucosal biopsy specimens were determined in 102 consecutive dyspeptic patients. In a separate group of 60 patients, rates of detection of Helicobacter pylori by culture of antral brushings and the length ofincubation required in selective and nonselective culture media were studied. In the first group of 102 patients, the combination of culture and histologic examination detected 54 Helicobacter pylori-positive patients, whereas the separate techniques each detected 51 Helicobacter pylori-positive patients. In the second group of 60 patients evaluated by culture of antral brushings, the rate of detection of Helicobacter pylori was 25 of 60 and was similar for culture (25/60) and histologic examination (25/60). In the second group the length of incubation required to detect Helicobacter pylori was different for selective andnonselective media, In nonselective media, incubation of up to ten days was required to detect all Helicobacter pylori infections, whereas in selective media seven days was sufficient. Rates of detection of Helicobacter pylori by culture, histopathologic examination and culture from brushings were similar, whereas the combination of culture and histopathologic examination achieved a superior rate of detection. The incubation period required for the detection of Helicobacter pylori by culture was a minimum of seven days and was dependent on the culture medium used.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:08:23