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Titolo:
THE TACHYKININ NK1 RECEPTOR MEDIATES THE MIGRATION-PROMOTING EFFECT OF SUBSTANCE-P ON HUMAN SKIN FIBROBLASTS IN CULTURE
Autore:
PARENTI A; AMERINI S; LEDDA F; MAGGI CA; ZICHE M;
Indirizzi:
UNIV FLORENCE,DEPT PHARMACOL,VIALE MORGAGNI 65 I-50134 FLORENCE ITALY UNIV FLORENCE,DEPT PHARMACOL I-50134 FLORENCE ITALY PHARMACOL DEPT MENARINI SRL I-50134 FLORENCE ITALY
Titolo Testata:
Naunyn-Schmiedeberg's archives of pharmacology
fascicolo: 5, volume: 353, anno: 1996,
pagine: 475 - 481
SICI:
0028-1298(1996)353:5<475:TTNRMT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; NONPEPTIDE ANTAGONIST; SENSORY NEURONS; STIMULATION; CAPSAICIN; RATS; EXPRESSION; INVIVO;
Keywords:
CELL MIGRATION; SUBSTANCE P; NK1 RECEPTOR; HUMAN SKIN FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
A. Parenti et al., "THE TACHYKININ NK1 RECEPTOR MEDIATES THE MIGRATION-PROMOTING EFFECT OF SUBSTANCE-P ON HUMAN SKIN FIBROBLASTS IN CULTURE", Naunyn-Schmiedeberg's archives of pharmacology, 353(5), 1996, pp. 475-481

Abstract

Fibroblast migration is an important component of the tissue responseduring the repair process, and substance P (SP) has been shown to exert trophic effects. In the present study, cell migration was evaluatedas the distance travelled by adherent human skin fibroblasts (HF) at 96 h and by the number of individual cells moving across a filter within 5 h. In control conditions (1% calf serum) adherent fibroblasts moved from the starting line by approximately 700 mu m. The addition of SP (10(-11)-10(-7) M) increased HF mobilisation in a concentration-dependent manner, with maximal activity at 10(-8) M (50% increase in migration over control). Migration of individual HF in suspension was also promoted by SP in a concentration-dependent manner, with an EC(50) Of 2.2x10(-9) M. The response produced by the maximally effective concentration of SP was equal to 65 and 90% of the effect elicited by 100 ng/ml Platelet-Derived Growth Factor A/B (PDGF A/B) on adherent and individual cells respectively. The synthetic NK1 receptor agonist [Sar(9)]SP-sulphone (10(-11)-10(-6) M) reproduced the SP effect. The NK2 and NK3 receptor agonists [beta A1a(8)]NKA(4-10) and [MePhe(7)]NKB were devoid of any effect. The effect of SP was antagonised by two selective antagonists of NK1 receptors, namely (+/-)CP 96,345 (10(-10)-10(-8) M) and FK 888 (10(-9)-10(-7) M), while the NK2 receptor antagonist MEN 10627 (10(-8)-10(-7) M) was not effective. Our data indicate that SP is apotent effector of fibroblast migration and the NK1 receptor is responsible for this effect. These observations further support the specific role of the NK1 receptor in mediating the trophic function of SP at the cutaneous level.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:08:21