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Titolo:
DETECTION OF GERM CELL-DERIVED PROTEINS IN TESTICULAR INTERSTITIAL FLUID - POTENTIAL FOR MONITORING SPERMATOGENESIS IN-VIVO
Autore:
TURNER KJ; MCKINNELL C; MCLAREN TT; QURESHI SJ; SAUNDERS PTK; FOSTER PMD; SHARPE RM;
Indirizzi:
MRC,REPROD BIOL UNIT,37 CHALMERS ST EDINBURGH EH3 9EW MIDLOTHIAN SCOTLAND MRC,REPROD BIOL UNIT EDINBURGH EH3 9EW MIDLOTHIAN SCOTLAND ZENECA CENT TOXICOL LAB MACCLESFIELD CHESHIRE ENGLAND
Titolo Testata:
Journal of andrology
fascicolo: 2, volume: 17, anno: 1996,
pagine: 127 - 136
SICI:
0196-3635(1996)17:2<127:DOGCPI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT TESTIS; SHARES IMMUNODETERMINANTS; LEYDIG-CELLS; TESTOSTERONE; INHIBIN; BINDING; PLASMA; BLOOD; IDENTIFICATION; TESTIBUMIN;
Keywords:
SEMINIFEROUS TUBULE-SECRETED PROTEINS; PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN; ARP-2; SPERMATID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
K.J. Turner et al., "DETECTION OF GERM CELL-DERIVED PROTEINS IN TESTICULAR INTERSTITIAL FLUID - POTENTIAL FOR MONITORING SPERMATOGENESIS IN-VIVO", Journal of andrology, 17(2), 1996, pp. 127-136

Abstract

The aim of the present study was to assess whether proteins secreted by the seminiferous tubules (ST) can be detected in testicular interstitial fluid (IF) and testicular (IV), spermatic (SV), and peripheral venous (PV) plasma from adult rats. An antiserum was raised against seminiferous tubule-conditioned medium (STCM) prepared from adult rats and used in conjunction with Western blot analysis to screen IF and blood samples resolved by one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Samples of IF and PV were analyzed from control adult rats and rats exposed to scrotal heating (43 degrees C for 30 minutes) 24 hours earlier to ascertain whether damage to spermatogenesis would affect 'leakage' of proteins from the seminiferous tubules. In all control rats, the STCM antiserum specifically detected three proteins in testicular IF with molecular weights of 24, 16, and 14 kDa, respectively. Heat treatment increased the abundance of these proteins and induced the appearance of several other less-abundant proteins, all with molecular masses below 25 kDa. Two of the proteins present in IF were identified, the 24-kDa protein as phosphatidylethanolamine-binding protein (PEEP), and the 14-kDa protein as an androgen-regulated protein (ARP-2). Both of these proteins have been shown in previous studies to be secreted by round spermatids. Our results suggest that germ cell secretory products can gain access to the interstitium under both normal physiological conditions and more easily after induction of damage to spermatogenesis. The antiserum was unable to detect any ST-derived proteins in blood, although it is likely that this result may be due to insensitivity of the presently used techniques, The development of specific immunoassays for germ cell-secreted proteins (e.g., PEEP and ARP-2) should enablemore definitive assessment of whether proteins secreted by the seminiferous epithelium can be measured in blood and thus provide a potential means of monitoring spermatogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:42:51