Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CRYOPROTECTANT TOXICITY IN PENAEID PRAWN EMBRYOS
Autore:
NEWTON SS; SUBRAMONIAM T;
Indirizzi:
UNIV NOTRE DAME,DEPT BIOL SCI NOTRE DAME IN 46556 UNIV MADRAS,DEPT ZOOL,UNIT INVERTEBRATE REPROD MADRAS 600025 TAMIL NADU INDIA
Titolo Testata:
Cryobiology
fascicolo: 1, volume: 33, anno: 1996,
pagine: 172 - 177
SICI:
0011-2240(1996)33:1<172:CTIPPE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DROSOPHILA EMBRYOS; CRYOPRESERVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
S.S. Newton e T. Subramoniam, "CRYOPROTECTANT TOXICITY IN PENAEID PRAWN EMBRYOS", Cryobiology, 33(1), 1996, pp. 172-177

Abstract

Cryopreservation of penaeid prawn embryos has definite applications in the aquaculture industry. It can be considered a viable alternative to solve the ''seed'' scarcity faced during the ''lean'' season. presently, there is no protocol for the cryopreservation of prawn embryos. As a study of cryoprotectant toxicity is an essential prerequisite forthe development of a cryopreservation protocol, this study focuses onthe toxicity of seven well known permeating, low molecular weight cryoprotectants: glycerol, formamide, acetamide, methanol, propylene glycol, dimethyl sulfoxide (Me(2)SO), and ethylene glycol (EG). In the cryoprotectant toxicity experiments embryos were exposed to cryoprotectant solutions at 15 degrees C for a period of 15 min, whereas the toxicity neutralization experiments were conducted at 28 degrees C with an exposure time of 15 min. The widely used permeating cryoprotectant, glycerol, was toxic to morula stage embryos at concentrations >0.5 M. This study shows that the toxicity tolerance of Penaeus indicus embryos varies with the developmental stage, the later stages being more resistant. Morulae did not tolerate any cryoprotectant above 5 M, whereas the nauplii had survival rates of 45% in 12.5 M methanol and 78% in 6.4 M EG. The toxicity neutralization experiments do not seem to indicate any specific toxicity-blocking mechanism in Me(2)SO toxicity reductionby EG. Based on the response to cryoprotectant toxicity, nauplius stage larvae of penaeid prawns can be considered a suitable stage for cryopreservation, both by the conventional slow cooling method and also the vitrification technique as it allows use of high levels of cryoprotectant. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:34:49