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Titolo:
INHIBITION OF HIV-1 IN CELL-CULTURE BY SYNTHETIC HUMATE ANALOGS DERIVED FROM HYDROQUINONE - MECHANISM OF INHIBITION
Autore:
SCHNEIDER J; WEIS R; MANNER C; KARY B; WERNER A; SEUBERT BJ; RIEDE UN;
Indirizzi:
UNIV FREIBURG,INST MED MIKROBIOL,ABT VIROL,HERMANN HERDER STR 11 D-79104 FREIBURG GERMANY PAUL EHRLICH INST D-63225 LANGEN GERMANY WEYL GMBH D-68305 MANNHEIM GERMANY UNIV FREIBURG,INST PATHOL D-79104 FREIBURG GERMANY
Titolo Testata:
Virology
fascicolo: 2, volume: 218, anno: 1996,
pagine: 389 - 395
SICI:
0042-6822(1996)218:2<389:IOHICB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; NEUTRALIZING MONOCLONAL-ANTIBODY; RECOMBINANT SOLUBLE CD4; ENVELOPE GLYCOPROTEIN; SULFATED POLYSACCHARIDES; TYPE-1; INFECTION; GP120; PHENOTYPE; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J. Schneider et al., "INHIBITION OF HIV-1 IN CELL-CULTURE BY SYNTHETIC HUMATE ANALOGS DERIVED FROM HYDROQUINONE - MECHANISM OF INHIBITION", Virology, 218(2), 1996, pp. 389-395

Abstract

Humic acids are natural constituents of soil and ground water and mainly consist of mixtures of polycyclic phenolic compounds. A similar complex of compounds with a mean size of about 1000 Da, designated HS-1500, was synthesized by oxidation of hydroquinone. HS-1500 inhibited HIV-1 infection of MT-2 cells with an IC50 of 50-300 ng/ml and showed a mean cell toxicity of about 600 mu g/ml. Inhibition of HIV-induced syncytium formation was observed at 10-50 mu g/ml. Treatment of free and cell-attached HIV with HS-1500 irreversibly reduced its infectivity, whereas the susceptibility of target cells for the virus was not impaired by treatment prior to infection. The HIV envelope protein gp120SU bound to sepharose-coupled HS-1500 and could be eluted by high salt anddetergent. HS-1500 interfered with the CD4-induced proteolytic cleavage of the vs loop of virion gp120SU. Furthermore, binding of V3 loop-specific antibodies was irreversibly inhibited, whereas binding of soluble GD4 to gp120SU on virus and infected cells was not affected. In conclusion, our data suggest, that the synthetic humic acid analogue inhibits the infectivity of HIV particles by interference with a V3 loop-mediated step of virus entry. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:36:23