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Titolo:
A COMBINED POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ENZYME ASSAY FOR DISCRIMINATING BETWEEN CAMPYLOBACTER-COLI AND CAMPYLOBACTER-JEJUNI
Autore:
COMI G; PIPAN C; BOTTA G; COCOLIN L; CANTONI C; MANZANO M;
Indirizzi:
UNIV UDINE,FAC AGR,DIPARTIMENTO SCI ALIMENTI,VIA MARANGONI 97 I-33100UDINE ITALY UNIV UDINE,FAC MED,CATTEDRA MICROBIOL MED I-33100 UDINE ITALY UNIV MILAN,FAC MED VET,IST ISPEZ ALIMENTI ORIGINE ANIM MILAN ITALY
Titolo Testata:
FEMS immunology and medical microbiology
fascicolo: 1, volume: 16, anno: 1996,
pagine: 45 - 49
SICI:
0928-8244(1996)16:1<45:ACPCAR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDENTIFICATION; PRODUCTS; LARIDIS; PROBES; WATER;
Keywords:
CAMPYLOBACTER JEJUNI; CAMPYLOBACTER COLI; POLYMERASE CHAIN REACTION; RESTRICTION ENDONUCLEASE ENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
G. Comi et al., "A COMBINED POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ENZYME ASSAY FOR DISCRIMINATING BETWEEN CAMPYLOBACTER-COLI AND CAMPYLOBACTER-JEJUNI", FEMS immunology and medical microbiology, 16(1), 1996, pp. 45-49

Abstract

A combined polymerase chain reaction and restriction endonuclease (RE) enzyme assay was developed to discriminate between Campylobacter coli and Campylobacter jejuni. Amplimers of the FlaA gene obtained by PCRwere digested with AluI and HinfI to distinguish C, coli from C. jejuni. With AluI digestion C. jejuni-specific bands were observed at 110,140 and 160 bp and C. coli-specific bands at 293 and 147 bp. C. jejuni-specific bands of 349 and 109 bp were found by HinfI digestion but HinfI did not digest the FlaA amplimer of C. coli. This combined technique is fast and easy to perform, and distinguishes the two campylobacters unequivocally.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:25:48