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Titolo:
IMPROVEMENT OF THE PROCESS FOR SORBITOL PRODUCTION WITH ZYMOMONAS-MOBILIS IMMOBILIZED IN KAPPA-CARRAGEENAN
Autore:
JANG KH; JUNG SJ; CHANG HS; CHUN UH;
Indirizzi:
VICTORIA UNIV TECHNOL,CTR BIOPROC & FOOD TECHNOL,WERRIBEE CAMPUS,POB 14428,MCMC MELBOURNE VIC 800 AUSTRALIA KYUNG HEE UNIV,COLL IND,DEPT FOOD SCI & TECHNOL SUWON 449701 SOUTH KOREA
Titolo Testata:
Process biochemistry
fascicolo: 5, volume: 31, anno: 1996,
pagine: 485 - 492
SICI:
1359-5113(1996)31:5<485:IOTPFS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-FRUCTOSE OXIDOREDUCTASE; PERMEABILIZED CELLS; YEAST-CELLS; BEAN GUM; KINETICS; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
K.H. Jang et al., "IMPROVEMENT OF THE PROCESS FOR SORBITOL PRODUCTION WITH ZYMOMONAS-MOBILIS IMMOBILIZED IN KAPPA-CARRAGEENAN", Process biochemistry, 31(5), 1996, pp. 485-492

Abstract

The production of sorbitol by permeabilized cells of Zymomonas mobilis immobilised in kappa-carrageenan has been investigated. Permeabilised cells were treated with ceiyltrimethylammoniumbromide (CTAB) prior to immobilisation to cross-link the enzymes of the glucose-fructose oxidoreductase (GFOR). Rigidity of the immobilised beads was increased two-fold with 90% conversion efficiency by addition of 40% (w/v) polyols(glycerol 25 g+propylene glycol 15g) to 60% (w/v) distilled water containing 2.5% (w/v) kappa-carrageenan as final concentration prior to immobilisation. kappa-Carrageenan beads entrapping permeabilised cells were dried to improve bead rigidity and storage stability. During a semi-batch process for 72 h with dry beads, there was an improvement of the loss of enzyme activity (less than 10%). In a batch process, the K-m,K-fructose value for the free cells, wet beads and dry kappa-carrageenan beads were 71.7, 72.4 and 116.7 g litre(-1) respectively. Higherproductivity was obtained with a two-stage continuous packed bed reactor with both wet and dry kappa-carrageenan beads.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 03:07:18