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Titolo:
CHICKEN GUANYLATE-BINDING PROTEIN - CONSERVATION OF GTPASE ACTIVITY AND INDUCTION BY CYTOKINES
Autore:
SCHWEMMLE M; KASPERS B; IRION A; STAEHELI P; SCHULTZ U;
Indirizzi:
UNIV FREIBURG,DEPT VIROL,INST MED MIKROBIOL & HYG,ABT VIROL,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,DEPT VIROL,INST MED MIKROBIOL & HYG,ABT VIROL D-79008 FREIBURG GERMANY UNIV MUNICH,INST TIERPHYSIOL MUNICH GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 17, volume: 271, anno: 1996,
pagine: 10304 - 10308
SICI:
0021-9258(1996)271:17<10304:CGP-CO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERFERON; ESTABLISHMENT; SEQUENCE; GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
M. Schwemmle et al., "CHICKEN GUANYLATE-BINDING PROTEIN - CONSERVATION OF GTPASE ACTIVITY AND INDUCTION BY CYTOKINES", The Journal of biological chemistry, 271(17), 1996, pp. 10304-10308

Abstract

To gain further insights into the cytokine network of birds, we used polymerase chain reaction technology to clone a cDNA that codes for a chicken homolog of the interferon-induced guanylate-binding proteins (GBPs). In its N-terminal moiety, the 64-kDa chicken GBP contains two sequence blocks of 100 and 19 amino acids, respectively, that are about70% identical to mammalian GBPs. The first region includes two motifsof the canonical GTP-binding consensus element. The other parts of chicken GBP are poorly conserved, except for a CAAX motif at the extremeC terminus which might signal isoprenylation. Like mammalian GBPs, recombinant chicken GBP specifically bound to agarose-immobilized guanine nucleotides and hydrolyzed GTP to both GDP and GMP. Regulation by interferons was also conserved: chicken GBP RNA was barely detectable inuninduced chicken cells. Low GBP RNA levels were found in cells treated with type I interferon, whereas very high levels were observed in cells treated with supernatant of a chicken T cell line that secretes agamma-interferon-like activity. Together with recent phylogenetic studies of interferon genes, these results suggest that in spite of low sequence conservation, the various components of the avian interferon system are functionally well conserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:55:44