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Titolo:
CLONING AND SEQUENCE-ANALYSIS OF GENES-CODING FOR PARAMECIUM SECRETORY GRANULE (TRICHOCYST) PROTEINS - A UNIQUE PROTEIN FOLD FOR A FAMILY OF POLYPEPTIDES WITH DIFFERENT PRIMARY STRUCTURES
Autore:
GAUTIER MC; SPERLING L; MADEDDU L;
Indirizzi:
UNIV PARIS 06,CTR GENET MOLEC,CNRS F-91198 GIF SUR YVETTE FRANCE UNIV PARIS 06,CTR GENET MOLEC,CNRS F-91198 GIF SUR YVETTE FRANCE
Titolo Testata:
The Journal of biological chemistry
fascicolo: 17, volume: 271, anno: 1996,
pagine: 10247 - 10255
SICI:
0021-9258(1996)271:17<10247:CASOGF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARIANT SURFACE GLYCOPROTEINS; HELICAL COILED COILS; SECONDARY STRUCTURE; TRYPANOSOMA-BRUCEI; BIOCHEMICAL-CHARACTERIZATION; TETRAURELIA; CLEAVAGE; ELECTROPHORESIS; PREDICTION; MOTIF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Gautier et al., "CLONING AND SEQUENCE-ANALYSIS OF GENES-CODING FOR PARAMECIUM SECRETORY GRANULE (TRICHOCYST) PROTEINS - A UNIQUE PROTEIN FOLD FOR A FAMILY OF POLYPEPTIDES WITH DIFFERENT PRIMARY STRUCTURES", The Journal of biological chemistry, 271(17), 1996, pp. 10247-10255

Abstract

The architecturally complex secretory granules of Paramecium, known as trichocysts, have two unusual and seemingly contradictory features: their protein contents have crystalline organization (Sperling, L., Tardieu, A., and Gulik-Krzywicki, T. (1987) J. Cell Biol. 105, 1649-1662), yet these proteins are a heterogeneous set of molecules encoded by a large multigene family (Madeddu, L., Gautier, M.-C., Vayssie, L., Houari, A., and Sperling, L. (1995) Mol. Biol. Cell 6, 649-659). We present here the first complete sequences of three genes coding for three different precursors of the trichocyst crystalline matrix proteins. The deduced protein sequences indicate that each precursor gives rise totwo of the mature polypeptides found in the crystalline trichocyst matrix. Analysis of putative processing sites suggests that a series of reactions, some of which may involve a novel endopeptidase, are involved in their proteolytic maturation. Each of the 6 mature polypeptides contains heptad segments. Characterization of the heptad segments leads us to propose that the mature polypeptides that compose the crystalline trichocyst matrix, despite their different primary structures, allshare a unique protein fold, probably a 4 alpha-helical antiparallel bundle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 14:21:47