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Titolo:
STRAIN DIFFERENTIATION OF ONCHOCERCA-VOLVULUS FROM UGANDA USING DNA PROBES
Autore:
FISCHER P; BAMUHIIGA J; KILIAN AHD; BUTTNER CW;
Indirizzi:
BERNHARD NOCHT INST TROP MED,DEPT HELMINTHOL & ENTOMOL,BERNHARD NOCHTSTR 74 D-20359 HAMBURG GERMANY GTZ FT PORTAL UGANDA MINIST HLTH,BASIC HLTH SERV FT PORTAL UGANDA
Titolo Testata:
Parasitology
, volume: 112, anno: 1996,
parte:, 4
pagine: 401 - 408
SICI:
0031-1820(1996)112:<401:SDOOFU>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAIN-FOREST; OCULAR ONCHOCERCIASIS; SIMULIUM-YAHENSE; SEQUENCE; INFECTION; SAVANNA; INTENSITY; COMMUNITY; SANCTIPAULI; PARASITE;
Keywords:
ONCHOCERCA VOLVULUS; POLYMERASE CHAIN REACTION; SOUTHERN BLOT; FOREST STRAIN-SPECIFIC DNA PROBE; MICROFILARIAL LENGTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
P. Fischer et al., "STRAIN DIFFERENTIATION OF ONCHOCERCA-VOLVULUS FROM UGANDA USING DNA PROBES", Parasitology, 112, 1996, pp. 401-408

Abstract

Polymerase chain reaction (PCR) combined with non-radioactive DNA hybridization was applied for the detection and characterization of a 150bp tandem repeat of Onchocerca volvulus. DNA of worms from western Uganda was amplified and then probed with a digoxygenin-labelled oligonucleotide, specific for the forest form of O. volvulus and compared to samples from various African countries. Hybridization was only observed with PCR products from the forest in Liberia, south-eastern Ghana, Benin and southern Cameroon, but not with worms from Uganda or the savannah in Burkina Faso and northern Ghana. A nested PCR using primers derived form the forest form-specific DNA sequence confirmed these results. Morphometric studies revealed length differences between the microfilariae of Ugandan O. volvulus to those of West Africa, especially tothose of the savannah in Burkina Faso. It is concluded that the forest/savannah classification of O. volvulus from West Africa is not suitable for Simulium neavei-transmitted O. volvulus from Uganda.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 11:05:36