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Titolo:
HISTOCHEMICAL EVALUATION OF PLACENTAL ANGIOTENSINASE-A IN PREECLAMPSIA - ENZYME-ACTIVITY IN VILLOUS TROPHOBLAST INDICATES AN ENHANCED LIKELIHOOD OF GESTATIONAL PROTEINURIC HYPERTENSION
Autore:
NEUDECK H; SCHUSTER C; HILDEBRANDT R; ONEY T; STIEMER B; HOPP H; GRAF R;
Indirizzi:
FREE UNIV BERLIN,INST ANAT,KONIGIN LUISE STR 15 D-14195 BERLIN GERMANY FREE UNIV BERLIN,INST TOXIKOL & EMBRYOPHARMAKOL D-14195 BERLIN GERMANY FREE UNIV BERLIN,UNIV KLINIKUM BENJAMIN FRANKLIN,FRAUENKLIN & POLIKLIN D-12200 BERLIN GERMANY
Titolo Testata:
Placenta
fascicolo: 2-3, volume: 17, anno: 1996,
pagine: 155 - 163
SICI:
0143-4004(1996)17:2-3<155:HEOPAI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINOPEPTIDASE-A; RENIN; PREGNANCY; SERUM; RESISTANCE; SUBSTRATE; RECEPTORS; DISEASE; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
H. Neudeck et al., "HISTOCHEMICAL EVALUATION OF PLACENTAL ANGIOTENSINASE-A IN PREECLAMPSIA - ENZYME-ACTIVITY IN VILLOUS TROPHOBLAST INDICATES AN ENHANCED LIKELIHOOD OF GESTATIONAL PROTEINURIC HYPERTENSION", Placenta, 17(2-3), 1996, pp. 155-163

Abstract

The aim of this study was to examine whether differences in placentalangiotensinase A (glutamyl aminopeptidase, EC 3.4.11.7) activities occurred in hypertensive complications of pregnancy compared with uncomplicated pregnancies. Biochemical and semiquantitative histochemical methods were used and compared for their applicability. Angiotensinase Aactivity was detected using L-alpha glutamyl-4-methoxy-2-naphthylamide (alpha-Glu-MNA) as substrate and Fast Blue B salt for simultaneous ate-coupling in cryostat sections of placental tissue samples from 32 patients with pre-eclampsia, 11 patients with pregnancy-induced hypertension and 44 participants with uncomplicated pregnancies. The graduated intensity of reaction product in the villous trophoblast and in fetal blood vessels was evaluated semiquantitatively in a double-blind study by light microscopy (semiquantitative score method). Score levels were related to relative frequencies of hypertensive disorders (proportional odds model) and correlated to the severity of gestational hypertension (Spearman's rank correlation). After detection of enzyme activity the same tissue samples were homogenized and used for kinetic fluorometric measurements under the same substrate and buffer conditions asin enzyme histochemistry. Enhanced villous trophoblastic angiotensinase A activity was significantly associated with an increased frequencyof pre-eclampsia in pregnant women (cumulative odds ratio x theta(1)=6.37; P<0.001) and showed significant correlations with the severity of gestational hypertensive disorders, represented by systolic (r=0.31;P<0.05) and diastolic (r=0.34; P<0.05) blood pressure and by concomitant proteinuria (r=0.44; P<0.01). Histochemical evaluation of fetal blood vessels and biochemical measurements revealed no statistically significant results. In conclusion this study demonstrates for the first time that increased villous trophoblastic angiotensinase A activity indicates an increased likelihood of the presence of pre-eclampsia and the severity of hypertensive disorders in pregnancy. (C) 1996 W. B. Saunders Company Ltd

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 02:02:03