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Titolo:
CHARACTERIZATION OF BACTERIAL-CELL MEMBRANE ATTACHMENT SITES OF PLASMID R6K
Autore:
JEMILOHUN PF; CLARK CW; ARCHIBOLD ER;
Indirizzi:
JACKSON STATE UNIV,DEPT BIOL JACKSON MS 39217 MOREHOUSE COLL,DEPT BIOL ATLANTA GA 30314
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 1, volume: 221, anno: 1996,
pagine: 186 - 192
SICI:
0006-291X(1996)221:1<186:COBMAS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACILLUS-SUBTILIS; ESCHERICHIA-COLI; DNA-REPLICATION; INVITRO BINDING; OUTER-MEMBRANE; ORIGIN; PROTEIN; ASSOCIATION; FRACTION; REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
P.F. Jemilohun et al., "CHARACTERIZATION OF BACTERIAL-CELL MEMBRANE ATTACHMENT SITES OF PLASMID R6K", Biochemical and biophysical research communications, 221(1), 1996, pp. 186-192

Abstract

In vitro binding studies revealed that plasmid R6K could attach to both inner and outer membrane fractions of its host cell, Escherichia coli. Derivatives of R6K carrying one or two of its three origins of replication could not bind stably to the same membrane fractions in the presence of salt. However, the derivative, pRK35, carrying the intact three origins of replication could bind stably to membrane fractions from its host in the presence or absence of salt. These observations suggest that the three origins of DNA replication must be contiguous for stable binding of the plasmid to the cell membrane. The results of binding experiments showed that plasmid R6K bound competitively with pRK35 as well as the heterologous plasmid, p1524. (C) 1996 Academic Press,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:25:55