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Titolo:
DIFFERENTIAL AND PERSISTENT EXPRESSION PATTERNS OF CNS GENE-TRANSFER BY AN ADENOASSOCIATED VIRUS (AAV) VECTOR
Autore:
MCCOWN TJ; XIAO X; LI J; BREESE GR; SAMULSKI RJ;
Indirizzi:
UNIV N CAROLINA,BIOL SCI RES CTR 223,CAMPUS BOX 7250 CHAPEL HILL NC 27599 UNIV N CAROLINA,BRAIN & DEV RES CTR CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT PHARMACOL CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT PSYCHIAT CHAPEL HILL NC 27599 UNIV N CAROLINA,GENE THERAPY CTR CHAPEL HILL NC 27599
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 713, anno: 1996,
pagine: 99 - 107
SICI:
0006-8993(1996)713:1-2<99:DAPEPO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFERIOR COLLICULUS; SEIZURE GENESIS; BRAIN; RAT;
Keywords:
GENE THERAPY; VIRAL VECTOR; ADENOASSOCIATED VIRUS; INFERIOR COLLICULUS; HIPPOCAMPUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
T.J. Mccown et al., "DIFFERENTIAL AND PERSISTENT EXPRESSION PATTERNS OF CNS GENE-TRANSFER BY AN ADENOASSOCIATED VIRUS (AAV) VECTOR", Brain research, 713(1-2), 1996, pp. 99-107

Abstract

Safe, long-term gene expression is a primary criteria for effective gene therapy in the brain, so studies were initiated to evaluate adeno-associated virus (AAV) vector transfer of a reporter gene into specific sites of the rat brain. In the 4 day old rat, site infusions of AAV-CMV-lacZ (1 mu l; 5 X 10(4) particles) produced neuronal beta-galactosidase gene expression 3 weeks later in the hippocampus and inferior colliculus, but not in the cerebral cortex. Seven days after infusion ofAAV-CMV-lacZ viral vectors (1 mu l) in the adult rat, beta-galactosidase gene expression was found in the olfactory tubercle, caudate, hippocampus, piriform cortex and inferior colliculus, primarily in multipolar neurons close to the infusion site. Three months after vector microinfusion, similar levels of gene expression remained in the olfactorytubercle and the inferior colliculus, with some reduction found in the caudate, but substantial reductions in beta-galactosidase gene expression occurred in the hippocampus and piriform cortex. In no case wereobvious signs of toxicity noted. Therefore, AAV vectors can transfer foreign genes into the adult and neonatal CNS, but the pattern and longevity of gene expression depends upon the area of brain being studied.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/08/20 alle ore 07:47:10