Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
OVEREXPRESSION OF A P-GLYCOPROTEIN IN HEPATOCELLULAR CARCINOMAS FROM WOODCHUCK HEPATITIS VIRUS-INFECTED WOODCHUCKS (MARMOTA-MONAX)
Autore:
DUNN SE; HUGHES CS; LEBLANC GA; CULLEN JM;
Indirizzi:
N CAROLINA STATE UNIV,COLL VET MED,DEPT MICROBIOL PATHOL & PARASITOL,4700 HILLSBOROUGH ST RALEIGH NC 27606 N CAROLINA STATE UNIV,COLL VET MED,DEPT MICROBIOL PATHOL & PARASITOL RALEIGH NC 27606 N CAROLINA STATE UNIV,COLL VET MED,DEPT TOXICOL RALEIGH NC 27695 N CAROLINA STATE UNIV,COLL VET MED,DEPT ANAT PHYSIOL SCI & RADIOL RALEIGH NC 27695
Titolo Testata:
Hepatology
fascicolo: 4, volume: 23, anno: 1996,
pagine: 662 - 668
SICI:
0270-9139(1996)23:4<662:OOAPIH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESISTANCE GENE-EXPRESSION; MULTIDRUG-RESISTANCE; SURFACE-ANTIGEN; RAT-LIVER; CANCER; CELLS; HEPATOCARCINOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Dunn et al., "OVEREXPRESSION OF A P-GLYCOPROTEIN IN HEPATOCELLULAR CARCINOMAS FROM WOODCHUCK HEPATITIS VIRUS-INFECTED WOODCHUCKS (MARMOTA-MONAX)", Hepatology, 23(4), 1996, pp. 662-668

Abstract

The leading cause of human hepatocellular carcinomas (HCCs) is hepatitis B virus (HBV) infection. Woodchucks infected with a closely related hepadnavirus, woodchuck hepatitis virus (WHV), serve as a model for HBV because woodchucks chronically infected with WHV also develop hepatocellular carcinomas. Increased expression of p-glycoprotein (pgp) inhuman HCCs is a common obstacle in successful cancer chemotherapy. Pgps are encoded by a family of multidrug-resistance (MDR) genes. Liversfrom uninfected and WHV-infected woodchucks were examined to determine if pgp was expressed in HCCs and if there was a difference in expression between HCCs and nonneoplastic liver. A 170-kd protein was identified by Western blot in HCCs, whereas, constitutive pgp was not detected in normal liver taken from the same animals in 3 of 3 cases. Immunolocalization of the pgp with a panel of monoclonal antibodies revealedintensification of staining in 7 of 20 foci and 12 of 22 HCCs from six animals. Using primers for the human MDR1 gene, a single product wasdetected by reverse-transcribed polymerase chain reaction (RT-PCR) from HCCs, We have shown an increase in pgp in HCCs compared with normalliver from WHV-infected woodchucks. This is the first example of the induction of a pgp in a naturally hepadnavirus infected rodent system. It suggests the woodchuck can be a useful model for the study of the acquisition of resistance to chemotherapeutic agents in virally induced HCCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 02:11:01