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Titolo:
SYNERGISTIC ENHANCEMENT OF BOTH INITIATION AND ELONGATION BY ACIDIC TRANSCRIPTION ACTIVATION DOMAINS
Autore:
BLAIR WS; FRIDELL RA; CULLEN BR;
Indirizzi:
DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,ROOM 426 CARL BLDG,RES DR DURHAM NC 27710 DUKE UNIV,MED CTR,HOWARD HUGHES MED INST DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT GENET DURHAM NC 27710
Titolo Testata:
EMBO journal
fascicolo: 7, volume: 15, anno: 1996,
pagine: 1658 - 1665
SICI:
0261-4189(1996)15:7<1658:SEOBIA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIV-1 TAT; FUNCTIONAL-ANALYSIS; TRANS-ACTIVATOR; MESSENGER-RNA; DNA-BINDING; EXPRESSION; ELEMENTS; SEQUENCE; PROTEIN; VIRUS;
Keywords:
ELONGATION; MESSENGER-RNA SYNTHESIS; TAT; TRANSCRIPTIONAL ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
W.S. Blair et al., "SYNERGISTIC ENHANCEMENT OF BOTH INITIATION AND ELONGATION BY ACIDIC TRANSCRIPTION ACTIVATION DOMAINS", EMBO journal, 15(7), 1996, pp. 1658-1665

Abstract

The effects of activation domain synergy on transcription initiation and elongation have been examined utilizing a system that permits the targeting of a defined number of activation modules to promoter DNA. As predicted, incremental increases in targeted activation potential were found to result in corresponding increases in transcription initiation. Surprisingly, however, transcriptional processivity, and hence mRNA synthesis, required a threshold level of activation domain synergy that exceeded the level required for at least modest levels of transcription initiation. The degree to which transcriptional processivity was enhanced was shown to depend on the quantity of activation modules targeted to the promoter DNA, rather than the quality. While the RNA-sequence specific HIV-1 Tat trans-activator was also shown to enhance processivity in this assay system, Tat differed from DNA-sequence specific activation domains in exerting a more dramatic effect on the efficiency of transcript elongation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:00:08