Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ABUNDANCE AND LENGTH POLYMORPHISM OF MICROSATELLITE REPEATS IN BETA-VULGARIS L
Autore:
MORCHEN M; CUGUEN J; MICHAELIS G; HANNI C; SAUMITOULAPRADE P;
Indirizzi:
UNIV LILLE 1,LAB GENET & EVOLUT POPULAT VEGETALES,URA CNRS 1185,BAT SN2 F-59655 VILLENEUVE DASCQ FRANCE UNIV LILLE 1,LAB GENET & EVOLUT POPULAT VEGETALES,URA CNRS 1185 F-59655 VILLENEUVE DASCQ FRANCE UNIV LILLE 1,INST AGR & ALIMENTAIRE LILLE F-59655 VILLENEUVE DASCQ FRANCE UNIV DUSSELDORF,INST BOT D-40225 DUSSELDORF GERMANY INST PASTEUR,URA CNRS 1160,UNITE ONCOL MOLEC F-59019 LILLE FRANCE
Titolo Testata:
Theoretical and Applied Genetics
fascicolo: 3-4, volume: 92, anno: 1996,
pagine: 326 - 333
SICI:
0040-5752(1996)92:3-4<326:AALPOM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA POLYMORPHISMS; SIMPLE SEQUENCES; SATELLITE DNAS; HUMAN GENOME; LINKAGE MAP; MARKERS; GENE; EVOLUTION; PCR; AMPLIFICATION;
Keywords:
VNTR; SIMPLE SEQUENCE LENGTH POLYMORPHISM; MICROSATELLITE; SEQUENCE-TAGGED SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M. Morchen et al., "ABUNDANCE AND LENGTH POLYMORPHISM OF MICROSATELLITE REPEATS IN BETA-VULGARIS L", Theoretical and Applied Genetics, 92(3-4), 1996, pp. 326-333

Abstract

Simple sequence repeats (SSRs) are known to exhibit high degrees of variability even among closely related individuals. Their usage as nuclear genetic markers requires their conversion into sequence-tagged sites (STSs). In this paper we present the development of simple sequences as STSs for Beta vulgaris. This species comprises wild, cultivated, and weedy forms; the latter are thought to originate from accidental hybridisation between the other two. Two partial genomic libraries werescreened with simple sequence motifs (AT, CA, CT, ATT, GTG, and CA, CT, respectively). Clones of 22 CA, nine CT, eight ATT, and one GTG sequence were obtained. AT microsatellites were present in compound motifs, not recognised by the probe. Sequence comparisons revealed that 20 CA clones containing short motifs (< 16 bp) were variants of a previously described approximately 320-bp satellite DNA (Schmidt et al. 1991), and hence did not correspond to unique loci. Polymorphism of one (ATT)(15) and three (CT)(n), with n = 15, 17 and 26, was detected by PCR on a sample of 64 plants from the different forms of B. vulgaris. 13 (ATT), 13 (CT), nine (CT) alleles and one (CT) allele were detected. One of the ATT alleles was much larger than the others (> 800 bp). Genetic variability was high among wild beets, lower among cultivated beets, and intermediate among weed beets. One allele of each locus was found at high frequencies in cultivated beets and, to a lower extent, in weed beets. The combination of three polymorphic loci allowed the individual identification of 17/17 wild and 15/15 weed beets, and 21/32, mostly homozygous, cultivated beets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 04:28:20