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Titolo:
RAPID SELECTION FOR AN N-LINKED OLIGOSACCHARIDE BY MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE V3 LOOP OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1
Autore:
SCHONNING K; JANSSON B; OLOFSSON S; HANSEN JES;
Indirizzi:
HVIDOVRE UNIV HOSP,INFECT DIS LAB,DEPT 144,KETTEGARD ALLE 30 DK-2650 HVIDOVRE DENMARK HVIDOVRE UNIV HOSP,INFECT DIS LAB DK-2650 HVIDOVRE DENMARK GOTHENBURG UNIV,DEPT CLIN VIROL S-41346 GOTHENBURG SWEDEN
Titolo Testata:
Journal of General Virology
, volume: 77, anno: 1996,
parte:, 4
pagine: 753 - 758
SICI:
0022-1317(1996)77:<753:RSFANO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUTRALIZATION EPITOPE; HTLV-III/LAV; GLYCOSYLATION; EVOLUTION; SEQUENCE; ENVELOPE; INFECTION; ANTIGENS; PLAQUE; GP120;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
K. Schonning et al., "RAPID SELECTION FOR AN N-LINKED OLIGOSACCHARIDE BY MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE V3 LOOP OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1", Journal of General Virology, 77, 1996, pp. 753-758

Abstract

The V3 loop of the human immunodeficiency virus (HIV) surface protein, gp120, constitutes a principal neutralizing determinant. HIV strainslacking a naturally conserved N-linked oligosaccharide (at position 306) within the V3 loop are highly sensitive to neutralization. We subjected molecular clones of HIVLAI, lacking this N-306-glycan to in vitro immune selection with MAbs directed against the V3 loop. In all, tenclones were characterized, and all proved resistant to V3-directed neutralization. Sequencing of the V3 loop revealed that six of the clones had become resistant at least partly by reacquisition of the N-306-glycan. Only two of the clones possessed mutations within the binding site of the antibody itself, while the two remaining clones did not display changes within the V3 loop itself. Thus, HIV strains lacking the N-306-glycan primarily develop resistance to V3-directed neutralization through acquisition of the specific oligosaccharide. This demonstrates that protein glycosylation can be a primary modifier of virus antigenicity of possible importance for the interaction of HN with the hostimmune response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:53:53