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Titolo:
CHONDROITIN-6-SULFATE INCORPORATED INTO COLLAGEN GELS FOR THE GROWTH OF HUMAN KERATINOCYTES - THE EFFECT OF CROSS-LINKING AGENTS AND DIAMINES
Autore:
HANTHAMRONGWIT M; REID WH; GRANT MH;
Indirizzi:
UNIV STRATHCLYDE,BIOENGN UNIT,106 ROTTENROW GLASGOW G4 0NW LANARK SCOTLAND UNIV STRATHCLYDE,BIOENGN UNIT GLASGOW G4 0NW LANARK SCOTLAND GLASGOW ROYAL INFIRM,BURNS UNIT GLASGOW LANARK SCOTLAND
Titolo Testata:
Biomaterials
fascicolo: 8, volume: 17, anno: 1996,
pagine: 775 - 780
SICI:
0142-9612(1996)17:8<775:CIICGF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPIDERMAL-KERATINOCYTES; ARTIFICIAL SKIN; FIBROBLASTS; INVITRO; TISSUE; DIFFERENTIATION; GLUTARALDEHYDE; STABILIZATION; BIOMATERIALS; SUBSTITUTE;
Keywords:
ARTIFICIAL SKIN; COLLAGEN; CHONDROITIN-6-SULFATE; PUTRESCINE-INDUCED CELL PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M. Hanthamrongwit et al., "CHONDROITIN-6-SULFATE INCORPORATED INTO COLLAGEN GELS FOR THE GROWTH OF HUMAN KERATINOCYTES - THE EFFECT OF CROSS-LINKING AGENTS AND DIAMINES", Biomaterials, 17(8), 1996, pp. 775-780

Abstract

This study demonstrates the effect of the glycosaminoglycans, hyaluronic acid and chondroitin-6-sulphate (Ch6SO(4)), diamines and a carbodiimide cross-linking agent on the growth of human epidermal cells on collagen gels. Ch6SO(4) incorporated into collagen gels stimulated cell growth rate, but the effect was found to be inconsistent. We found that approximately 50% of the incorporated Ch6SO(4) in the gels leached out into the growth medium after the first 3 d in culture, and this is thought to lead to the inconsistent cell growth response. In order to minimize the elution of Ch6SO(4) from the gels and thereby maximize its effect on the growth of the keratinocytes, 1-100 mu g ml(-1) Ch6SO(4) was added in the medium. The results showed that Ch6SO(4) at these concentrations in the medium did not stimulate the cell growth on either plain collagen gels or gels containing 20% Ch6SO(4). As an alternative strategy, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and diamines (putrescine or diaminohexane) were used to immobilize Ch6SO(4) ontothe collagen gels and to cross-link the gels. The cross-linking process partially prevented the elution of Ch6SO(4) from the gels. Interestingly, only putrescine, not diaminohexane, promoted the growth of keratinocytes on the cross-linked plain collagen gels. We proposed to develop an artificial skin substitute containing putrescine as a growth factor for the human epidermal cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 09:34:05