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Titolo:
HYPEROXIA-RESPONSIVE PROTEINS IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL-CELLS
Autore:
VISNER GA; FOGG S; NICK HS;
Indirizzi:
UNIV FLORIDA,DEPT PEDIAT,BOX 100296 GAINESVILLE FL 32610 UNIV FLORIDA,DEPT BIOCHEM GAINESVILLE FL 32610 UNIV FLORIDA,DEPT MOLEC BIOL GAINESVILLE FL 32610
Titolo Testata:
American journal of physiology. Lung cellular and molecular physiology
fascicolo: 4, volume: 14, anno: 1996,
pagine: 517 - 525
SICI:
1040-0605(1996)14:4<517:HPIRPM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPEROXIDE-DISMUTASE; HEME OXYGENASE; TOXICITY; INJURY; GENE; LUNG; EXPRESSION; ACTIVATION; PROTECTION; ENDOTOXIN;
Keywords:
2-DIMENSIONAL PROTEIN GEL ELECTROPHORESIS; HEME OXYGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
G.A. Visner et al., "HYPEROXIA-RESPONSIVE PROTEINS IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL-CELLS", American journal of physiology. Lung cellular and molecular physiology, 14(4), 1996, pp. 517-525

Abstract

Exposure to high partial pressures of oxygen are toxic to the lung, and much of the damage observed is related to injury of the pulmonary microvasculature. In this study, ave evaluated the response of the pulmonary microvascular endothelial cell to high oxygen concentrations, using two-dimensional protein gel electrophoresis as a direct molecular assay of differences between cells exposed to room air or hyperoxia. We observed a differential expression of five specific proteins within 24 h of a hyperoxic insult that we termed hyperoxia-responsive proteins. After 4 h of hyperoxia there was a decrease in two of the proteins. From 8 to 24 h we observed a repression of a third and an induction of the other two proteins. One of the induced proteins was also increased by heat shock and hydrogen peroxide and has characteristics similarto heat shock protein (HSP) 32 (hemeoxygenase 1). Western analysis using an antibody specific to rat heme oxygenase 1 verified that this oxygen-responsive protein is heme oxygenase 1. The response of the otherfour hyperoxia-responsive proteins appears to be specific to oxygen and not a general stress response, since they were not changed in response to heat shock or hydrogen peroxide. Based on RNA inhibitor and pulse chase experiments, these changes may result from transcriptional/posttranscriptional mechanisms or hyperoxia-dependent protein turnover.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 04:02:09