Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SCLERODERMA FIBROBLAST PHENOTYPE IS MODULATED BY ENDOTHELIAL-CELL COCULTURE
Autore:
DENTON CP; XU SW; WELSH KI; PEARSON JD; BLACK CM;
Indirizzi:
ROYAL FREE HOSP,DEPT RHEUMATOL,RHEUMATOL UNIT,POND ST LONDON NW3 2QG ENGLAND ROYAL FREE HOSP,DEPT RHEUMATOL,RHEUMATOL UNIT LONDON NW3 2QG ENGLAND UNIV LONDON KINGS COLL,VASC BIOL RES CTR LONDON WC2R 2LS ENGLAND CHURCHILL HOSP,DEPT TRANSPLANTAT IMMUNOL OXFORD OX3 7LJ ENGLAND
Titolo Testata:
Journal of rheumatology
fascicolo: 4, volume: 23, anno: 1996,
pagine: 633 - 638
SICI:
0315-162X(1996)23:4<633:SFPIMB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRESSIVE SYSTEMIC-SCLEROSIS; GROWTH-FACTORS; DERMAL FIBROBLASTS; COLLAGEN; TISSUE; SCAR;
Keywords:
SCLERODERMA; FIBROBLAST; ENDOTHELIAL CELL; COCULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
C.P. Denton et al., "SCLERODERMA FIBROBLAST PHENOTYPE IS MODULATED BY ENDOTHELIAL-CELL COCULTURE", Journal of rheumatology, 23(4), 1996, pp. 633-638

Abstract

Objective. We established a co-culture system to investigate endothelial cell-fibroblast interaction in scleroderma (systemic sclerosis, SSc). Such a system allows reciprocal interaction between these cells. The pattern of phenotypic modulation for normal and SSc fibroblasts in co-culture was compared. Methods. A virally transformed human umbilical vein endothelial cell (HUVEC) derived cell line (1E-7) was cultured on nitrocellulose membrane inserts above dermal fibroblast monolayers. The effect of co-culture on fibroblast number, [H-3]-thymidine ([H-3]-TdR) incorporation, and collagen (type I) production were compared for 10 SSc and 5 control cell lines. Co-culture with the epithelial lines A549 and A431, and nontransformed HUVEC, was also investigated. Results. We observed a statistically significant increase in cell number and a reduction in collagen production for SSc, but not control, fibroblasts co-cultured with endothelial cells. This co-culture also promoted [H-3]-TdR incorporation in both SSc and control fibroblasts. While epithelial cell lines did not influence fibroblast cell number, collagen production by SSc fibroblasts was diminished by A549. Conclusion. Endothelial cell derived soluble factors modulate fibroblast properties in co-culture, and the different response of SSc compared with normal fibroblasts provides further evidence for a link between endothelial and fibroblast dysfunction in this disease. However, similar effects onSSc fibroblast collagen production were also observed for some epithelial cells, suggesting that modulation of fibroblast properties is notrestricted to cells of endothelial origin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:18:21