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Titolo:
THE ELONGATING RIBOSOME - STRUCTURAL AND FUNCTIONAL-ASPECTS
Autore:
NIERHAUS KH; BEYER D; DABROWSKI M; SCHAFER MA; SPAHN CMT; WADZACK J; BITTNER JU; BURKHARDT N; DIEDRICH G; JUNEMANN R; KAMP D; VOSS H; STUHRMANN HB;
Indirizzi:
MAX PLANCK INST MOLEC GENET,AG RIBOSOMEN,IHNESTR 73 D-14195 BERLIN GERMANY GKSS FORSCHUNGSZENTRUM GEESTHACHT GMBH D-21502 GEESTHACHT GERMANY
Titolo Testata:
Biochemistry and cell biology
fascicolo: 11-12, volume: 73, anno: 1995,
pagine: 1011 - 1021
SICI:
0829-8211(1995)73:11-12<1011:TER-SA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFER-RNA-BINDING; ESCHERICHIA-COLI RIBOSOMES; ALLOSTERIC 3-SITE MODEL; AMINOACYL-TRANSFER RNA; E-SITE; CYCLE; TRANSLATION; MUTAGENESIS;
Keywords:
TRANSFER-RNA-RIBOSOMAL CONTACTS; NEUTRON SCATTERING; LOCATION OF TRANSFER-RNAS IN RIBOSOMES; MOVEMENT OF RIBOSOME ON MESSENGER-RNA; PEPTIDYLTRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
K.H. Nierhaus et al., "THE ELONGATING RIBOSOME - STRUCTURAL AND FUNCTIONAL-ASPECTS", Biochemistry and cell biology, 73(11-12), 1995, pp. 1011-1021

Abstract

We determined the positions and arrangements of RNA ligands within the ribosome with a new neutron-scattering technique, the proton-spin contrast-variation. Two tRNAs were bound to the ribosome in the pre-translocational and the post-translocational state. The mass centre of gravity of both tRNAs resides at the subunit interface of the body of the30S subunit. Both tRNAs are separated by an angle of 50-55 degrees, and their mutual arrangement does not change during translocation. The mass centre of gravity moves by 13 +/- 3 Angstrom (1 Angstrom = 0.1 nm) during translocation, corresponding well with the length of one codon. Using an RNase-digestion technique, the length of the mRNA sequencecovered by the ribosome was determined to be 39 +/- 3 nucleotides before and after translocation. The ribosome moves like a rigid frame along the mRNA during translocation. In contrast, both tRNAs seem to be located on a movable ribosomal domain, which carries the tRNAs before, during, and after translocation, leaving the microtopography of the tRNAs with the ribosome unaltered. This conclusion was derived from an analysis of the contract patterns of thioated tRNAs on the ribosome. The results have led to a new model of the elongation cycle, which reinterprets the features of the previous ''allosteric three-sites model'' in a surprisingly simple fashion. Finally, a mutational analysis has identified a single nucleotide of the 23S rRNA essential for the peptidyltransferase activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:37:01