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Titolo:
INHIBITION OF DOPAMINE SYNTHESIS BY DOPAMINE D2 AND D3 BUT NOT D4 RECEPTORS
Autore:
OHARA CM; UHLANDSMITH A; OMALLEY KL; TODD RD;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT,4940 CHILDRENS PL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT GENET ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT ANAT & NEUROBIOL ST LOUIS MO 63110
Titolo Testata:
The Journal of pharmacology and experimental therapeutics
fascicolo: 1, volume: 277, anno: 1996,
pagine: 186 - 192
SICI:
0022-3565(1996)277:1<186:IODSBD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT TYROSINE-HYDROXYLASE; AUTORECEPTOR-MEDIATED INHIBITION; PROTEIN KINASE-II; PERTUSSIS TOXIN; PREFRONTAL CORTEX; MESSENGER-RNA; BRAIN-SLICES; PHOSPHORYLATION; NEURONS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Ohara et al., "INHIBITION OF DOPAMINE SYNTHESIS BY DOPAMINE D2 AND D3 BUT NOT D4 RECEPTORS", The Journal of pharmacology and experimental therapeutics, 277(1), 1996, pp. 186-192

Abstract

The goal of the current study was to determine which of the De-like receptors (D2, D3 or D4) are involved in autoreceptor regulation of dopamine synthesis. We have derived a model system utilizing a mouse mesencephalic cell line, MN9D, which both synthesizes and releases dopamine, to characterize the modulation of tyrosine hydroxylase activity, the rate limiting enzyme in the conversion of tyrosine to dopamine, by the D2-like receptors. Previously, we have shown that stimulation of D2and D3, but not D4, dopamine receptors transfected into MN9D cells inhibited the release of dopamine. In the current study, we show that quinpirole stimulation of transfected D2 and D3, but not D4, dopamine receptors inhibited K+-stimulated tyrosine hydroxylase activity in a pertussis toxin-sensitive manner, strongly suggesting G-protein coupling as a mechanistic pathway. The D2 receptor effect could be maintained for at least 60 min, whereas the D3 receptor effect desensitized. Treatment with 10 mu M forskolin, which raises cyclic AMP levels, or with 100 nM okadaic acid, a potent phosphatase inhibitor, had no effect on the D2- or D3-mediated inhibition, suggesting that these effects may beindependent of both cyclic AMP- and okadaic acid-sensitive phosphatase activity. Taken together, these data confirm the hypothesis that dopamine D2 and D3 receptors can perform dual roles in autoreceptor regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:51:43