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Titolo:
APOPTOSIS IS AN EARLY EVENT DURING PHTHALOCYANINE PHOTODYNAMIC THERAPY-INDUCED ABLATION OF CHEMICALLY-INDUCED SQUAMOUS PAPILLOMAS IN MOUSE SKIN
Autore:
AGARWAL R; KORMAN NJ; MOHAN RR; FEYES DK; JAWED S; ZAIM MT; MUKHTAR H;
Indirizzi:
CASE WESTERN RESERVE UNIV,UNIV HOSP CLEVELAND,SKIN DIS RES CTR,DEPT DERMATOL,2074 ABINGTON RD CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,UNIV HOSP CLEVELAND,SKIN DIS RES CTR,DEPT DERMATOL CLEVELAND OH 44106
Titolo Testata:
Photochemistry and photobiology
fascicolo: 4, volume: 63, anno: 1996,
pagine: 547 - 552
SICI:
0031-8655(1996)63:4<547:AIAEED>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; HEPATIC MICROSOMES; DNA FRAGMENTATION; LYMPHOMA-CELLS; PHOTOSENSITIZERS; TETRASULFONATE; MECHANISMS; ACTIVATION; INVITRO; TUMORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
R. Agarwal et al., "APOPTOSIS IS AN EARLY EVENT DURING PHTHALOCYANINE PHOTODYNAMIC THERAPY-INDUCED ABLATION OF CHEMICALLY-INDUCED SQUAMOUS PAPILLOMAS IN MOUSE SKIN", Photochemistry and photobiology, 63(4), 1996, pp. 547-552

Abstract

Photodynamic therapy (PDT) is a promising new modality to treat malignant neoplasms including superficial skin cancers, In our search for an ideal photosensitizer for PDT, Pc 4, a silicon phthalocyanine, has shown promising results both in in vitro assays and in implanted tumors, In this study we assessed the efficacy of Pc 4 PDT in the ablation of murine skin tumors; and the evidence for apoptosis during tumor ablation was also obtained, The Pc 4 was administered through tail vein injection to SENCAR mice bearing chemically induced squamous papillomas,and 24 h later the lesions were illuminated with an argon ion-pumped dye laser tuned at 675 nm for a total light dose of 135 J/cm(2), Within 72-96 h, almost complete tumor shrinkage occurred; no tumor regrowthwas observed up to 90 days post-PDT, As evident by nucleosome-size DNA fragmentation, appearance of apoptotic bodies in hematoxylin and eosin staining and direct immunoperoxidase detection of digoxigenin-labeled genomic DNA in sections, apoptosis was clearly evident 6 h post-PDTat which time tumor shrinkage was less than 30%, The apoptotic bodies, as evident by the condensation of chromatin material around the periphery of the nucleus and increased vacuolization of the cytoplasm, were also observed in electron microscopic studies of the tumor tissues following Pc 4 PDT, The extent of apoptosis was greater at 15 h than at6 and 10 h post-PDT, Taken together, our results clearly show that Pc4 may be an effective photosensitizer for PDT of nonmelanoma skin cancer, and that apoptosis is an early event during this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:52:19