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Titolo:
ROLE FOR THE BETA(2) INTEGRIN CD11B IN MEDIATING EXPERIMENTAL LUNG INJURY IN MICE
Autore:
MIOTLA JM; WILLIAMS TJ; HELLEWELL PG; JEFFERY PK;
Indirizzi:
ROYAL BROMPTON NATL HEART & LUNG HOSP,DEPT LUNG PATHOL,SYDNEY ST LONDON SW3 6NP ENGLAND ROYAL BROMPTON NATL HEART & LUNG HOSP,DEPT LUNG PATHOL LONDON SW3 6NPENGLAND ROYAL BROMPTON NATL HEART & LUNG HOSP,DEPT APPL PHARMACOL LONDON SW3 6NP ENGLAND
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 4, volume: 14, anno: 1996,
pagine: 363 - 373
SICI:
1044-1549(1996)14:4<363:RFTBIC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULMONARY VASCULAR SEQUESTRATION; TYPE-3 COMPLEMENT RECEPTOR; NEUTROPHIL SEQUESTRATION; POLYMORPHONUCLEAR LEUKOCYTES; MYELOMONOCYTIC CELLS; MONOCLONAL-ANTIBODY; CHEMOTACTIC FACTORS; ADHESION MOLECULES; PERMEABILITY; INFLAMMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Miotla et al., "ROLE FOR THE BETA(2) INTEGRIN CD11B IN MEDIATING EXPERIMENTAL LUNG INJURY IN MICE", American journal of respiratory cell and molecular biology, 14(4), 1996, pp. 363-373

Abstract

We have investigated the requirement of neutrophil emigration and therole for CD11b/CD18-mediated events in the experimental induction of acute lung injury. BALB/c mice received lipopolysaccharide (LPS) (3 mg/kg) intravenously (i.v.) 2 h prior to i.v. zymosan (10 mg/kg) and extravascular albumin accumulation was assessed after 30 min. Compared with saline-treated controls, zymosan alone caused a 6-fold increase in the accumulation of I-125-human serum albumin in whole lung tissue (P < 0.05). Combined treatment with LPS and zymosan further increased extravascular albumin accumulation (P < 0.05 compared with zymosan alone). The monoclonal antibody 5C6, directed against murine CD11b, was injected, 1 mg i.v., 15 min prior to LPS or 15 min before the zymosan, andcompared with immunoglobulin G-injected controls. Albumin accumulation was significantly reduced by 5C6 when given prior to the LPS (P < 0.01), but not when given before zymosan in the combined LPS and zymosantreatment. Interestingly, albumin accumulation induced by zymosan alone was not reduced by 5C6. The lungs of the mice treated with LPS and zymosan showed a marked, diffuse accumulation of inflammatory cells which, by light microscopy, appeared to be interstitial. Foci of neutrophil aggregates were seen in noncapillary microvessels, and pretreatment with 5C6 appeared to reduce their frequency. In the animals treated with zymosan alone, LPS alone, or LPS and zymosan in combination, electron microscopy established that approximately 25% of all nucleated cells were neutrophils: 99% of the neutrophils were restricted to the intravascular compartment. Pretreatment with 5C6 prior to LPS and zymosan treatment reduced the increase in percentage of neutrophils by half. These results indicate a disassociation between induction of permeability and neutrophil emigration in our murine model and suggest that the release of neutrophil-derived factors such as platelet-activating factor, proteases, or oxidants may be involved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:49:21