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Titolo:
CELLULAR MECHANISM OF ANGIOTENSIN-II-INDUCED ATRIAL-NATRIURETIC-PEPTIDE RELEASE IN RAT RIGHT ATRIAL TISSUE
Autore:
SOUALMIA H; MASSON F; BARTHELEMY C; MAISTRE G; CARAYON A;
Indirizzi:
CHU PITIE SALPETRIERE,SERV BIOCHIM MED,91 BD HOP F-75634 PARIS 13 FRANCE
Titolo Testata:
Life sciences
fascicolo: 19, volume: 58, anno: 1996,
pagine: 1621 - 1629
SICI:
0024-3205(1996)58:19<1621:CMOAA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL TRISPHOSPHATE; HEART-FAILURE; SECRETION; RESPONSES; CARDIOMYOCYTES; MYOCYTES; INFUSION; STRETCH; INVITRO;
Keywords:
ANGIOTENSIN II; ATRIAL NATRIURETIC PEPTIDE; LOSARTAN; INTRACELLULAR CALCIUM; PROTEIN KINASE C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
H. Soualmia et al., "CELLULAR MECHANISM OF ANGIOTENSIN-II-INDUCED ATRIAL-NATRIURETIC-PEPTIDE RELEASE IN RAT RIGHT ATRIAL TISSUE", Life sciences, 58(19), 1996, pp. 1621-1629

Abstract

This study presents an investigation of the mechanism of angiotensin II (Ang II)-induced atrial natriuretic peptide (ANP) release in superfused sliced right atria of rats. Ang II (0.1 mu M) enhanced ANP release by 49 %. This phenomenon was significantly blocked by (Sara(1)-Ileu(8)) Ang II (1 mu M) and losartan (0.1 mu M) The use of neomycin (100 mu M), a phospholipase-C inhibitor completely suppressed the effect of Ang II on ANP increase. To elucidate the intracellular mechanism of ANP released by Ang II, the role of protein kinase C (PKC) was determined by 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) and phorbol ester : 4-beta-phorbol 12-myristate-13-acetate (PMA). We observed that PMA (0.1 mu M) stimulated ANP release whereas H-7 (10 mu M), an inhibitor of PKC in the presence of Ang II, prevented ANP increase. The roleof calcium was also evaluated with 8-(N-N-diethylamino)-ocytyl-3,4,5,trimethoxy-benzoate (TMB-8) (10 mu M) and N-(6-aminohexyl)-5-chloro-1-naphtalene sulfonamide (W-7) (10 mu M), which completely inhibited ANP release by Ang II. Pre-treatment with diltiazem (10 mu M), an antagonist of the Ca++ channel, did not prevent ANP increase due to Ang II, but A23187 (5 mu M) enhanced ANP release by Ang II. These results suggest that PKC and intracellular calcium play an important role in ANP release under the influence of Ang II in rat atrial tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:16:38