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Titolo:
CONTRACTILE AND METABOLIC EFFECTS OF INCREASED CREATINE-KINASE ACTIVITY IN MOUSE SKELETAL-MUSCLE
Autore:
ROMAN BB; FOLEY JM; MEYER RA; KORETSKY AP;
Indirizzi:
CARNEGIE MELLON UNIV,DEPT BIOL SCI,4400 5TH AVE PITTSBURGH PA 15213 CARNEGIE MELLON UNIV,DEPT BIOL SCI PITTSBURGH PA 15213 CARNEGIE MELLON UNIV,PITTSBURGH NMR CTR BIOMED RES PITTSBURGH PA 15213 MICHIGAN STATE UNIV,DEPT PHYS EDUC & EXERCISE SCI E LANSING MI 48824 MICHIGAN STATE UNIV,DEPT PHYSIOL E LANSING MI 48824 MICHIGAN STATE UNIV,DEPT RADIOL E LANSING MI 48824
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 4, volume: 39, anno: 1996,
pagine: 1236 - 1245
SICI:
0363-6143(1996)39:4<1236:CAMEOI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED P-31 NMR; RAT MUSCLE; KINETIC-PROPERTIES; PHOSPHOCREATINE; PHOSPHOKINASE; SHUTTLE; INVIVO; FIBERS; HEART; LOCALIZATION;
Keywords:
ISOMETRIC CONTRACTION; TRANSGENIC; P-31 NUCLEAR MAGNETIC RESONANCE; PHOSPHOCREATINE; MYOSIN ISOFORM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
B.B. Roman et al., "CONTRACTILE AND METABOLIC EFFECTS OF INCREASED CREATINE-KINASE ACTIVITY IN MOUSE SKELETAL-MUSCLE", American journal of physiology. Cell physiology, 39(4), 1996, pp. 1236-1245

Abstract

The effects of increased expression of creatine kinase (CK) in skeletal muscle were studied in control and transgenic animals homozygous for expression of the B subunit of CK. CK activity was 47% higher in transgenic gastrocnemius muscle. The CK activity was distributed as follows: 45 +/- 1% MM dimer, 31 +/- 4% MB dimer, and 22 +/- 5% BB dimer. Nosignificant differences in metabolic or contractile proteins were detected except for a 22% decrease in lactate dehydrogenase activity and a 9% decrease in adenylate kinase activity. The only significant effect in contractile activity was that the rise time of a 5-s isometric contraction was 28% faster in the transgenic muscle. P-31 nuclear magnetic resonance (NMR) spectra were obtained from control and transgenic muscles during mechanical activation, and there were no NMR measurable differences detected. These results indicate that a 50% increase in CKactivity due to expression of the B subunit does not have large effects on skeletal muscle metabolism or contractile function. Therefore, control muscle has sufficient CK activity to keep up with changes in cellular high-energy phosphates except during the early phase of intensecontractile activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 05:59:41