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Titolo:
PREDOMINANT EXPRESSION OF THE LONG ISOFORM OF BCL-X (BCL-XL) IN HUMANLYMPHOMAS
Autore:
XERRI L; PARC P; BROUSSET P; SCHLAIFER D; HASSOUN J; REED JC; KRAJEWSKI S; BIRNBAUM D;
Indirizzi:
INST PAOLI CALMETTES,DEPT PATHOL,232 BD ST MARGUERITE,BP 156 F-13273 MARSEILLE 9 FRANCE INST PAOLI CALMETTES,DEPT BIOL MOL TUMEURS F-13273 MARSEILLE 9 FRANCE CHU PURPAN TOULOUSE FRANCE U119 INSERM MARSEILLE FRANCE LA JOLLA CANC RES FDN LA JOLLA CA 92037
Titolo Testata:
British Journal of Haematology
fascicolo: 4, volume: 92, anno: 1996,
pagine: 900 - 906
SICI:
0007-1048(1996)92:4<900:PEOTLI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-DEATH; GENE; CLONING;
Keywords:
LYMPHOMA; BCL-XL; BCL-XS; RT-PCR; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
L. Xerri et al., "PREDOMINANT EXPRESSION OF THE LONG ISOFORM OF BCL-X (BCL-XL) IN HUMANLYMPHOMAS", British Journal of Haematology, 92(4), 1996, pp. 900-906

Abstract

Bcl-x is a member of the bcl-2 family of proteins which are characterized by their ability to modulate apoptosis. Alternative splicing results in two distinct bcl-x mRNAs encoding a long isoform, bcl-xL, whichacts as a bcl-2 agonist: and a short isoform, bcl-xS, which inhibits bcl-2 effects. The aim of the study Mras to determine whether bcl-x isexpressed in lymphoma tissues and to characterize the respective production of bcl-xS and bcl-xL, We investigated the expression of bcl-x mRNA in a series of 50 non-Hodgkin's lymphomas (NHL) and Hodgkin's disease (HD) cases using a RT-PCR method in order to amplify both transcripts simultaneously, and to estimate their relative abundance. The rearrangements of the bcl-2 gene were analysed by RT-PCR expression of thehybrid bcl-2-IgH mRNA, In addition, 20 PCR-positive NHL cases and three HD cases were analysed by immunohistochemistry using bcl-x polyclonal antisera. RT-PCR showed bcl-x expression in 43/45 NHLs and 5/5 HD cases, The bcl-xL transcript was predominant in all positive cases and was associated with variable amounts of bcl-xS. There was no significant correlation between the profile of bcl-xL/bcl-xS expression and thehistological and immunological subtyping. Bcl-x immunodetection was positive in the neoplastic cell component in all analysed cases, but the degree of staining was highly variable between cases, Expression of the hybrid bcl-2-IgH gene was detected by RT-PCR in fine cases of follicular NHL and in one case of HD, but this group of tumours did not display a particular profile of bcl-xL/bcl-xS expression, We conclude that bcl-x is commonly expressed by malignant cells in various types of malignant lymphomas. with a predominance of the bcl-xL transcript, Since the corresponding bcl-xL isoform can block the cell death machineryand potentialize bcl-2 effects, it may be involved in some pathways of lymphomagenesis.

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Documento generato il 24/11/20 alle ore 11:17:31