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Titolo:
DETAILED OLIGOSACCHARIDE STRUCTURES OF HUMAN INTEGRIN ALPHA-5-BETA-1 ANALYZED BY A 3-DIMENSIONAL MAPPING TECHNIQUE
Autore:
NAKAGAWA H; ZHENG MZ; HAKOMORI S; TSUKAMOTO Y; KAWAMURA Y; TAKAHASHI N;
Indirizzi:
NAKANO VINEGAR CO LTD,NAKANO CENT RES INST,GLYCOLAB,2-6 NAKAMURA CHO HANDA 475 JAPAN NAKANO VINEGAR CO LTD,NAKANO CENT RES INST,GLYCOLAB HANDA 475 JAPAN BIOMEMBRANE INST SEATTLE WA 98119 UNIV WASHINGTON,DEPT PATHOBIOL SEATTLE WA 98195 UNIV WASHINGTON,DEPT MICROBIOL SEATTLE WA 98195
Titolo Testata:
European journal of biochemistry
fascicolo: 1, volume: 237, anno: 1996,
pagine: 76 - 85
SICI:
0014-2956(1996)237:1<76:DOSOHI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN FIBRONECTIN RECEPTOR; SUGAR CHAINS; ADHESION; GLYCOSYLATION; FAMILY;
Keywords:
INTEGRIN ALPHA-5-BETA-1; HUMAN OLIGOSACCHARIDE STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H. Nakagawa et al., "DETAILED OLIGOSACCHARIDE STRUCTURES OF HUMAN INTEGRIN ALPHA-5-BETA-1 ANALYZED BY A 3-DIMENSIONAL MAPPING TECHNIQUE", European journal of biochemistry, 237(1), 1996, pp. 76-85

Abstract

Structures of N-linked oligosaccharides obtained from human integrin alpha 5 beta 1 are described. Integrin alpha 5 beta 1 (4.5 mg) was purified from human placenta and digested using trypsin and chymotrypsin. N-linked oligosaccharides were released from the glycopeptides by digestion with glycoamidase A (from almond). The reducing ends of the oligosaccharides were derivatized with 2-aminopyridine. The pyridylamino-oligosaccharides were separated and these structures were identified by a three-dimensional HPLC mapping technique on three kinds of HPLC columns [Takahashi, N., Nakagawa, H., Fujikawa, K., Kawamura, Y. & Tomiya, N. (1995) Anal. Biochem. 226, 139-146]. Finally, 35 different oligosaccharide structures were identified, 10 of which were neutral, 6 mono-sialyl, 10 di-sialyl, 7 tri-sialyl and 2 tetra-sialyl. The molar ratio of neutral, mono-sialyl, di-sialyl, tri-sialyl and tetra-sialyl oligosaccharides was 20.8%, 24.8%, 27.7%, 18.1% and 8.6%, respectively. High-mannose-type oligosaccharides accounted for only 1.5% of the total. The remaining oligosaccharides were all complex type. The most predominant structure was the diantennary di-alpha-(2,3)-sialyl fucosyl. Major linking of sialic acid was alpha-(2,3)-linkage, and over 50% of all oligosaccharides were fucosylated at the N-acetylglucosamine residueof the reducing end.

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Documento generato il 28/09/20 alle ore 15:37:15