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Titolo:
DEVELOPMENT OF COLONIC AND PANCREATIC ENDOCRINE TUMORS IN MICE EXPRESSING A GLUCAGON-SV40 T-ANTIGEN TRANSGENE
Autore:
ASA SL; LEE YC; DRUCKER DJ;
Indirizzi:
MT SINAI HOSP,DEPT PATHOL,600 UNIV AVE TORONTO ON M5G 1X5 CANADA UNIV TORONTO TORONTO ON M5G 1X5 CANADA UNIV TORONTO,BANTING & BEST DIABET CTR TORONTO ON M5G 1L5 CANADA TORONTO HOSP,DEPT MED TORONTO ON M5T 2S8 CANADA TORONTO HOSP,DEPT CLIN BIOCHEM TORONTO ON M5T 2S8 CANADA TORONTO HOSP,DEPT GENET TORONTO ON M5T 2S8 CANADA CHINESE UNIV HONG KONG,DEPT PHYSIOL SHA TIN NEW TERR HONG KONG
Titolo Testata:
Virchows Archiv
fascicolo: 6, volume: 427, anno: 1996,
pagine: 595 - 606
SICI:
0945-6317(1996)427:6<595:DOCAPE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELL TYPES; MOUSE SMALL INTESTINE; PEPTIDE-YY; GENE-EXPRESSION; GASTROINTESTINAL-TRACT; CARCINOID-TUMORS; INSULIN; GLICENTIN; ORIGIN; DIFFERENTIATION;
Keywords:
TRANSGENIC MICE; GLUCAGON; COLON CARCINOMA; PANCREATIC ENDOCRINE CARCINOMA; MORPHOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
S.L. Asa et al., "DEVELOPMENT OF COLONIC AND PANCREATIC ENDOCRINE TUMORS IN MICE EXPRESSING A GLUCAGON-SV40 T-ANTIGEN TRANSGENE", Virchows Archiv, 427(6), 1996, pp. 595-606

Abstract

We report the histological, immunohistochemical and ultrastructural changes in mice containing a chimeric glucagon-simian virus 40 T antigen (SV40Tag) gene. Transgene expression was detected in endocrine cellsof pancreas, small and large intestine. Hyperplasia of glucagon-containing cells developed in pancreas and large bowel by gestational day 19. In large bowel, hyper plastic cells increased in number postnatallyand invasive carcinomas were identified at 4 weeks; several animals had lymph node metastases. In contrast, no pathology was detected in the small bowel in any of the transgenic mice. Colonic tumours expressedSV40Tag, proglucagon-derived peptides and peptide YY (PYY); scatteredcells contained cholecystokinin or glycoprotein hormone cc-subunit. Somatostatin or serotonin was also detected in some tumours. By electron microscopy, the colonic tumours retained features of endocrine differentiation, but secretory granules were smaller than those of non-tumorous intestinal glucagon-producing L cells. In postnatal pancreas, atypical cells containing SV40Tag and glucagon were initially clustered at the periphery of islets; this atypical hyperplasia progressed to neoplasia by 11-12 weeks. Some neoplastic pancreatic cells contained glucagon, PYY or vasoactive intestinal peptide immunopositivity, but most were negative for all peptides; they contained immunoreactivity for tyrosine hydroxylase and by electron microscopy, pancreatic tumour cellshad neuronal features. Pancreatic polypeptide was not detected in thenon-tumorous islets of transgenic animals. This line of transgenic mice provides a model for the analysis of endocrine tumour progression in the gut and pancreas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:23:18