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Titolo:
LACK OF INTERMEDIATE-AFFINITY INTERLEUKIN-2 RECEPTOR IN MICE LEADS TODEPENDENCE ON INTERLEUKIN-2-RECEPTOR-ALPHA, INTERLEUKIN-2-RECEPTOR-BETA-AND-GAMMA CHAIN EXPRESSION FOR T-CELL GROWTH
Autore:
CHASTAGNER P; MOREAU JL; JACQUES Y; MIYASAKA M; KONDO M; SUGAMURA K; THEZE J;
Indirizzi:
INST PASTEUR,UNITE IMMUNOGENET CELLULAIRE,25 RUE DR ROUX F-75015 PARIS FRANCE INST PASTEUR,UNITE IMMUNOGENET CELLULAIRE F-75015 PARIS FRANCE INST BIOL,INSERM U211 NANTES FRANCE OSAKA UNIV,SCH MED,DEPT BIOREGULAT,BIOMED RES CTR SUITA OSAKA 565 JAPAN TOHOKU UNIV,SCH MED,DEPT MICROBIOL SENDAI MIYAGI 980 JAPAN
Titolo Testata:
European Journal of Immunology
fascicolo: 1, volume: 26, anno: 1996,
pagine: 201 - 206
SICI:
0014-2980(1996)26:1<201:LOIIRI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
IL-2 RECEPTOR; MONOCLONAL-ANTIBODY; MOUSE INTERLEUKIN-2; P55; COMPLEX; CLONING; LINE; GENE; TRANSCRIPTION; ANTAGONIST;
Keywords:
INTERLEUKIN-2; INTERLEUKIN-2 RECEPTOR ALPHA, BETA AND GAMMA CHAINS; T CELL ACTIVATION; T CELL PROLIFERATION; CYTOKINE RESPONSIVENESS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
P. Chastagner et al., "LACK OF INTERMEDIATE-AFFINITY INTERLEUKIN-2 RECEPTOR IN MICE LEADS TODEPENDENCE ON INTERLEUKIN-2-RECEPTOR-ALPHA, INTERLEUKIN-2-RECEPTOR-BETA-AND-GAMMA CHAIN EXPRESSION FOR T-CELL GROWTH", European Journal of Immunology, 26(1), 1996, pp. 201-206

Abstract

An interleukin (IL) 4 dependant mouse T cell clone 8.2 derived from an IL-2-dependent T cell line was characterized. As measured by flow cytometric analysis and Northern blotting, it expresses IL-2 receptor beta (IL-2R beta) and gamma (IL-2R gamma) chains, but has lost expression of IL-2 receptor alpha chain (IL-2R alpha). To investigate the properties of the mouse IL-2R beta gamma complex and the role of IL-2R alpha gene expression, this clone was further studied. T cell clone 8.2 has lost the capacity to bind I-125-labeled human IL-2 under experimental conditions able to detect intermediate-affinity IL-2R in human cells. Mouse IL-2 is unable to block the binding of mAb TM beta 1 to 8.2 cells. Under the same experimental conditions, mouse IL-2 blocks the binding of TM beta 1 to C30-1 cells expressing the IL-2 alpha beta gamma complex. Since TM beta 1 recognizes an epitope related to the IL-2 binding site of IL-2R beta. these results can be taken as a demonstrationthat mouse IL-2R beta gamma does not bind mouse IL-2. Furthermore, T cell clone 8.3 does nor proliferate in response to recombinant mouse or human IL-2. On the other hand, T cell transfectant lines expressing heterospecific receptors made of the human IL-2R beta and mouse IL-2R gamma chains bind I-125-labeled human IL-2 and proliferate in responseto IL-2. This establishes the difference between mouse and human IL-2R beta chains. Transfection of T cell clone 8.2 with human IL-2R alphagenes restores their capacity to proliferate in response to IL-2. In addition, all transfectants grown in IL-2 express the endogeneous mouse IL-2R alpha chain. When grown in IL-4, the endogeneous mouse IL-2R alpha gene remains silent in all these transfectants. These results show that, contrary to the human, the mouse does not express an intermediate-affinity IL-2R. Expression of the IL-2R alpha gene is therefore required for the formation of the functional IL-2R in mice.

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Documento generato il 26/01/20 alle ore 16:06:12