Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CATIONIC PROTEINS ALTER SMOOTH-MUSCLE FUNCTION BY AN EPITHELIUM-DEPENDENT MECHANISM
Autore:
COYLE AJ; MITZNER W; IRVIN CG;
Indirizzi:
NATL JEWISH CTR IMMUNOL & RESP MED,DEPT MED,PULM PHYSIOL UNIT,1400 JACKSON ST DENVER CO 80206 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT MED,PULM PHYSIOL UNIT,1400 JACKSON ST DENVER CO 80206 UNIV COLORADO,HLTH SCI CTR,DEPT MED,DIV PULM SCI DENVER CO 80262 JOHNS HOPKINS UNIV,DEPT ENVIRONM HLTH SCI,DIV ENVIRONM PHYSIOL BALTIMORE MD 21205
Titolo Testata:
Journal of applied physiology
fascicolo: 4, volume: 74, anno: 1993,
pagine: 1761 - 1768
SICI:
8750-7587(1993)74:4<1761:CPASFB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR BASIC-PROTEIN; PIG ISOLATED TRACHEALIS; INHIBITORY FACTOR; AIRWAY HYPERRESPONSIVENESS; BRONCHOALVEOLAR LAVAGE; MILD ASTHMA; EOSINOPHIL; MODULATION; HYPERREACTIVITY; RESPONSIVENESS;
Keywords:
EPITHELIUM; EPITHELIUM-DEPENDENT RELAXING FACTOR; TRACHEA; AIR-WAY HYPERRESPONSIVENESS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Coyle et al., "CATIONIC PROTEINS ALTER SMOOTH-MUSCLE FUNCTION BY AN EPITHELIUM-DEPENDENT MECHANISM", Journal of applied physiology, 74(4), 1993, pp. 1761-1768

Abstract

Using a perfused guinea pig tracheal tube preparation, which allows the selective application of agonists to either the serosal or luminal surface, we have investigated whether two synthetic cationic proteins,poly-L-arginine and poly-L-lysine, can modify epithelium-dependent responses. With an intact epithelium, methacholine was approximately 150times less potent when applied intraluminally than when applied extraluminally. This difference was abolished by chemically removing the epithelium with the detergent CHAPS. Intraluminal application of KCl induced a dose-related relaxation of a precontracted trachea, which was also abolished by epithelium removal. Perfusion of the luminal surface with cationic proteins for 1 h (10 mug/ml) increased the potency of intraluminally applied methacholine without modifying the responses to extraluminally applied methacholine. Cationic proteins also attenuated the relaxant effects of intraluminally applied KCl. These effects occurred in the absence of any overt epithelial cell damage. in contrast, when the serosal surface of the trachea was treated with poly-L-arginine, there was no modification of either methacholine-induced contraction or KCl-induced relaxation. The effects of poly-L-arginine were inhibited by coperfusion with the polyanions albumin (10 mug/ml) or heparin (100 mug/ml). In contrast to cationic proteins, intraluminal perfusion with a polyanion, poly-L-aspartate (10 mug/ml), failed to modify either methacholine-induced contraction or KCl-induced relaxation. Our data demonstrate that cationic proteins can modify epithelium-dependentresponses in the airways. Although the precise mechanisms are unclear, a role is suggested for a charge-mediated interaction with the respiratory epithelium, resulting in airway smooth musele dysfunction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:15:57