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Titolo:
DIMINISHED SOLUBLE AND TOTAL CELLULAR L-SELECTIN IN CORD-BLOOD IS ASSOCIATED WITH ITS IMPAIRED SHEDDING FROM ACTIVATED NEUTROPHILS
Autore:
KOENIG JM; SIMON J; ANDERSON DC; SMITH EO; SMITH CW;
Indirizzi:
TEXAS CHILDRENS HOSP,SECT LEUKOCYTE BIOL,CLIN CARE CTR SUITE 1130,6621 FANNIN HOUSTON TX 77030 TEXAS CHILDRENS HOSP,SECT NEONATOL HOUSTON TX 77030 BAYLOR COLL MED,CHILDRENS NUTR RES CTR,DEPT PEDIAT HOUSTON TX 77030
Titolo Testata:
Pediatric research
fascicolo: 4, volume: 39, anno: 1996,
parte:, 1
pagine: 616 - 621
SICI:
0031-3998(1996)39:4<616:DSATCL>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; LEUKOCYTE ADHESION DEFICIENCY; ENDOTHELIAL-CELLS INVITRO; NEONATAL NEUTROPHILS; TRANSENDOTHELIAL MIGRATION; POLYMORPHONUCLEAR LEUKOCYTES; CD18-INDEPENDENT ADHESION; CHEMOTACTIC FACTORS; PROGENITOR CELLS; GRANULOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Koenig et al., "DIMINISHED SOLUBLE AND TOTAL CELLULAR L-SELECTIN IN CORD-BLOOD IS ASSOCIATED WITH ITS IMPAIRED SHEDDING FROM ACTIVATED NEUTROPHILS", Pediatric research, 39(4), 1996, pp. 616-621

Abstract

We have previously shown that surface levels of the adhesive glycoprotein, L-selectin, are diminished on cord blood neutrophils (polymorphonuclear leukocytes, PMN) and associated with impaired adherence to endothelium under flow conditions. To test the hypothesis that diminishedsurface levels reflect a total cellular deficiency, we measured L-selectin in PMN lysates and plasma from cord and adult blood. L-selectin content was decreased in cord blood PMN lysates compared with those ofadults by both Western blot analyses and ELISA (cord blood, 1195 +/- 160 pg/mL; adult, 1870 +/- 260 pg/mL; X +/- SEM; p < 0.05). Soluble L-selectin levels were also decreased in cord blood plasma (324 +/- 24 ng/mL versus 537 +/- 28 ng/mL in adult plasma, p < 0.01). To evaluate L-selectin function, we next compared the dose dependent effect of several chemoattractants on shedding of L-selectin from cord blood and adult PMN. Adult PMN showed greater overall shedding of L-selectin as compared with cord blood PMN after stimulation with fMet-Leu-Phe (p < 0.03) and granulocyte-macrophage colony-stimulating factor (p < 0.02), Incontrast, shedding of L-selectin was similar between groups after IL-8 tested stimulation. We conclude that cord blood PMN have a decreasedcellular content of L-selectin in addition to an impaired ability to shed surface L-selectin in response to specific inflammatory mediators.

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Documento generato il 23/01/21 alle ore 01:58:25