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Titolo: DIMINISHED SOLUBLE AND TOTAL CELLULAR L-SELECTIN IN CORD-BLOOD IS ASSOCIATED WITH ITS IMPAIRED SHEDDING FROM ACTIVATED NEUTROPHILS
Autore: KOENIG JM; SIMON J; ANDERSON DC; SMITH EO; SMITH CW;
- Indirizzi:
- TEXAS CHILDRENS HOSP,SECT LEUKOCYTE BIOL,CLIN CARE CTR SUITE 1130,6621 FANNIN HOUSTON TX 77030 TEXAS CHILDRENS HOSP,SECT NEONATOL HOUSTON TX 77030 BAYLOR COLL MED,CHILDRENS NUTR RES CTR,DEPT PEDIAT HOUSTON TX 77030
- Titolo Testata:
- Pediatric research
fascicolo: 4,
volume: 39,
anno: 1996,
parte:, 1
pagine: 616 - 621
- SICI:
- 0031-3998(1996)39:4<616:DSATCL>2.0.ZU;2-F
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- COLONY-STIMULATING FACTOR; LEUKOCYTE ADHESION DEFICIENCY; ENDOTHELIAL-CELLS INVITRO; NEONATAL NEUTROPHILS; TRANSENDOTHELIAL MIGRATION; POLYMORPHONUCLEAR LEUKOCYTES; CD18-INDEPENDENT ADHESION; CHEMOTACTIC FACTORS; PROGENITOR CELLS; GRANULOCYTE;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Citazioni:
- 48
- Recensione:
- Indirizzi per estratti:
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- Citazione:
- J.M. Koenig et al., "DIMINISHED SOLUBLE AND TOTAL CELLULAR L-SELECTIN IN CORD-BLOOD IS ASSOCIATED WITH ITS IMPAIRED SHEDDING FROM ACTIVATED NEUTROPHILS", Pediatric research, 39(4), 1996, pp. 616-621
Abstract
We have previously shown that surface levels of the adhesive glycoprotein, L-selectin, are diminished on cord blood neutrophils (polymorphonuclear leukocytes, PMN) and associated with impaired adherence to endothelium under flow conditions. To test the hypothesis that diminishedsurface levels reflect a total cellular deficiency, we measured L-selectin in PMN lysates and plasma from cord and adult blood. L-selectin content was decreased in cord blood PMN lysates compared with those ofadults by both Western blot analyses and ELISA (cord blood, 1195 +/- 160 pg/mL; adult, 1870 +/- 260 pg/mL; X +/- SEM; p < 0.05). Soluble L-selectin levels were also decreased in cord blood plasma (324 +/- 24 ng/mL versus 537 +/- 28 ng/mL in adult plasma, p < 0.01). To evaluate L-selectin function, we next compared the dose dependent effect of several chemoattractants on shedding of L-selectin from cord blood and adult PMN. Adult PMN showed greater overall shedding of L-selectin as compared with cord blood PMN after stimulation with fMet-Leu-Phe (p < 0.03) and granulocyte-macrophage colony-stimulating factor (p < 0.02), Incontrast, shedding of L-selectin was similar between groups after IL-8 tested stimulation. We conclude that cord blood PMN have a decreasedcellular content of L-selectin in addition to an impaired ability to shed surface L-selectin in response to specific inflammatory mediators.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 01:58:25