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Titolo:
NITRIC-OXIDE DIFFUSION IN MEMBRANES DETERMINED BY FLUORESCENCE QUENCHING
Autore:
DENICOLA A; SOUZA JM; RADI R; LISSI E;
Indirizzi:
UNIV REPUBLICA,FAC MED,DEPT BIOQUIM,AVDA GRAL FLORES 2125 MONTEVIDEO 11800 URUGUAY UNIV REPUBLICA,FAC CIENCIAS,DEPT PHYS BIOCHEM MONTEVIDEO 11800 URUGUAY UNIV SANTIAGO CHILE,DEPT CHEM SANTIAGO CHILE
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 1, volume: 328, anno: 1996,
pagine: 208 - 212
SICI:
0003-9861(1996)328:1<208:NDIMDB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOSYNTHESIS; SUPEROXIDE; OXYGEN; PYRENE;
Keywords:
NITRIC OXIDE; DIFFUSION; FLUORESCENCE; FREE RADICALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Denicola et al., "NITRIC-OXIDE DIFFUSION IN MEMBRANES DETERMINED BY FLUORESCENCE QUENCHING", Archives of biochemistry and biophysics, 328(1), 1996, pp. 208-212

Abstract

Quenching of pyrene derivative fluorescence by nitric oxide was used to evaluate the apparent diffusion coefficients of nitric oxide in artificial and biological membranes. The apparent second-order quenching constants of nitric oxide were obtained from Stern-Volmer plots using methyl- and undecylpyrene derivatives incorporated into liposomes and erythrocyte plasma membranes in order to assess the ability of nitric oxide to interact with molecules located at different positions in themembrane. Diffusion coefficients were estimated from the determined second-order quenching constants and compared to that of oxygen obtained under the same conditions. Oxygen and nitric oxide presented similardiffusional behavior in agreement with their similarity in structures, with the differences observed attributable to the higher lipophilicity of oxygen compared to nitric oxide. In solution, both showed the same quenching efficiency while in liposomes and erythrocyte ghosts oxygen diffusion was twice that of nitric oxide (k(O2)/k(NO) = 2) Nitric oxide diffusion coefficients determined at 20 degrees C ranged from 1.3x 10(-5) cm(2) s(-1) in liposomes to 0.4 X 10(-5) cm(2) s(-1) in surface erythrocyte plasma membranes. Both nitric oxide and oxygen had larger quenching constants for the undecyl derivative compared to the methylpyrene compound incorporated into erythrocyte plasma membranes, indicating an increased solubility of both gases toward the center of themembrane. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:40:02