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Titolo:
PHYSICAL MAP OF THE GENOME OF PLANCTOMYCES-LIMNOPHILUS, A REPRESENTATIVE OF THE PHYLOGENETICALLY DISTINCT PLANCTOMYCETE LINEAGE
Autore:
WARDRAINEY N; RAINEY FA; WELLINGTON EMH; STACKEBRANDT E;
Indirizzi:
DEUTSCH SAMMLUNG MIKROORGANISMEN & ZELLKULTUREN G,MASCHERODER WEG 1B D-38124 BRAUNSCHWEIG GERMANY DEUTSCH SAMMLUNG MIKROORGANISMEN & ZELLKULTUREN G D-38124 BRAUNSCHWEIG GERMANY UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND
Titolo Testata:
Journal of bacteriology
fascicolo: 7, volume: 178, anno: 1996,
pagine: 1908 - 1913
SICI:
0021-9193(1996)178:7<1908:PMOTGO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIELD GEL-ELECTROPHORESIS; RESTRICTION MAP; CHLAMYDIA-TRACHOMATIS; MYCOPLASMA-PNEUMONIAE; RIBOSOMAL-RNA; RRN OPERONS; COLI; CONSTRUCTION; CHROMOSOME; BACTERIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
N. Wardrainey et al., "PHYSICAL MAP OF THE GENOME OF PLANCTOMYCES-LIMNOPHILUS, A REPRESENTATIVE OF THE PHYLOGENETICALLY DISTINCT PLANCTOMYCETE LINEAGE", Journal of bacteriology, 178(7), 1996, pp. 1908-1913

Abstract

A physical map of the chromosome of Planctomyces limnophilus DSM 3776(T) was constructed by pulsed-field gel electrophoresis techniques, A total of 32 cleavage sites for the rare cutting restriction endonucleases PacI, PmeI, and SwaI were located on the chromosome, which was shown to be circular and approximately 5.2 Mbp in size, An extrachromosomal element was detected but was found not to be cleaved by any of the enzymes used in the analysis of the chromosome, The order of the fragments on the chromosome was determined by hybridization of excised, labelled restriction fragments to Southern blots of pulsed-field gel electrophoresis-separated restriction digests, Seven genetic markers, rrs,rrl, atpD, tuf, gyrB, rpoD, and dnaK, on the chromosome were located by hybridization, Probes for all genetic markers were obtained by PCR,For five of these markers, probes were constructed by PCR with degenerate primers targeting conserved sequences, The arrangement of the genetic markers was compared with that found in other bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:05:49