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Titolo:
97-KDA LINEAR IGA BULLOUS DERMATOSIS (LAD) ANTIGEN LOCALIZES TO THE LAMINA-LUCIDA OF THE EPIDERMAL BASEMENT-MEMBRANE
Autore:
ISHIKO A; SHIMIZU H; MASUNAGA T; HASHIMOTO T; DMOCHOWSKI M; WOJNAROWSKA F; BHOGAL BS; BLACK MM; NISHIKAWA T;
Indirizzi:
KEIO UNIV,SCH MED,DEPT DERMATOL,SHINJUKU KU,35 SHINANOMACHI TOKYO 160JAPAN CHURCHILL HOSP,DEPT DERMATOL OXFORD OX3 7LJ ENGLAND ST JOHNS INST DERMATOL LONDON ENGLAND
Titolo Testata:
Journal of investigative dermatology
fascicolo: 4, volume: 106, anno: 1996,
pagine: 739 - 743
SICI:
0022-202X(1996)106:4<739:9LIBD(>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DERMATITIS-HERPETIFORMIS; ULTRASTRUCTURAL-LOCALIZATION; INDIRECT IMMUNOFLUORESCENCE; EPIDERMOLYSIS-BULLOSA; SKIN; CHILDHOOD; DISEASE; ANTIBODIES; DEPOSITS; ADULTS;
Keywords:
IMMUNOELECTRON MICROSCOPY; BULLOUS DISEASE; AUTOIMMUNE DISEASE; IMMUNOGOLD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A. Ishiko et al., "97-KDA LINEAR IGA BULLOUS DERMATOSIS (LAD) ANTIGEN LOCALIZES TO THE LAMINA-LUCIDA OF THE EPIDERMAL BASEMENT-MEMBRANE", Journal of investigative dermatology, 106(4), 1996, pp. 739-743

Abstract

Linear IgA bullous dermatosis (LAD) is an autoimmune blistering disease in which IgA autoantibodies develop against the epidermal basement membrane zone. Target antigens of the circulating autoantibodies are thought to be heterogeneous, and their ultrastructural localization hasnot been fully elucidated. Previous studies with immunoblotting have demonstrated that the 97-kDa autoantigen is detected most frequently in patients' sera and is thought to be a major LAD antigen. Although a recent report suggests that the 97-kDa antigen localized to the hemidesmosomal plaques and the adjacent lamina lucida, discrepancies still exist among previous immunoelectron microscopic findings. To identify the precise localization of the 97-kDa LAD antigen, we used two different low-temperature immunoelectron microscopic techniques. For immunolabeling, we selected five LAD sera that had a high titer of autoantibodies against the 97-kDa LAD antigen. A post-embedding method with cryofixation and freeze substitution failed to immunolabel the 97-kDa LAD antigen. Cryoultramicrotomy with immunoelectron microscopy succeeded inpreserving the antigenicity of the 97-kDa LAD antigen. In all cases, the majority of labeling occurred in the lamina lucida beneath the hemidesmosomes. No specific labeling was observed in the hemidesmosomal attachment plaques or the lamina densa or sublamina densa region, including anchoring fibrils. These results indicate that the 97-kDa LAD antigen is a component of the lamina lucida.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 18:46:09