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Titolo:
EXPRESSION OF FULL-LENGTH DESMOSOMAL GLYCOPROTEINS (DESMOCOLLINS) IS NOT SUFFICIENT TO CONFER STRONG ADHESION ON TRANSFECTED L929 CELLS
Autore:
CHIDGEY MAJ; CLARKE JP; GARROD DR;
Indirizzi:
UNIV MANCHESTER,SCH BIOL SCI,3239 STOPFORD BLDG,OXFORD RD MANCHESTER M13 9PT LANCS ENGLAND UNIV MANCHESTER,SCH BIOL SCI MANCHESTER M13 9PT LANCS ENGLAND
Titolo Testata:
Journal of investigative dermatology
fascicolo: 4, volume: 106, anno: 1996,
pagine: 689 - 695
SICI:
0022-202X(1996)106:4<689:EOFDG(>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOPLASMIC DOMAINS; MDCK CELLS; CADHERINS;
Keywords:
DESMOSOME; CADHERIN; CATENIN; CYTOSKELETON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M.A.J. Chidgey et al., "EXPRESSION OF FULL-LENGTH DESMOSOMAL GLYCOPROTEINS (DESMOCOLLINS) IS NOT SUFFICIENT TO CONFER STRONG ADHESION ON TRANSFECTED L929 CELLS", Journal of investigative dermatology, 106(4), 1996, pp. 689-695

Abstract

Desmocollins are cadherin-like glycoproteins that are localized in desmosomes. They are thought to play a role in cell adhesion but direct evidence for this is currently unavailable. For this reason we have expressed cDNAs encoding full-length bovine desmocollin type la and typeIb in mouse fibroblast (L929) cells. This system has Previously been used to demonstrate the adhesive properties of E-cadherin. F-cadherin-mediated cell-cell adhesion is thought to require interaction of the cytoplasmic domain with the catenins that are expressed in L-cells. Because L929 cells do not express cytoplasmic desmosomal components that may be required for desmocollin-mediated adhesion, we constructed a chimeric cDNA encoding the bovine type 1 extracellular domain linked to the mouse E-cadherin transmembrane and cytoplasmic domains. cDNAs weretransfected: into cells and clones that expressed heterologous protein at the cell surface were isolated. The full-length desmocollins apparently did not interact with any other molecules, but the chimeric protein did bind to endogenous mouse alpha- and beta-catenin. Surprisingly none of the desmocollin-transfected cell lines showed significant adhesive properties under conditions where cells transfected with F-cadherin exhibited strong adhesiveness. We conclude that desmocollin expression alone is not sufficient to confer adhesion on transfected cells and more than one desmosomal component may be required.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 11:16:26