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Titolo:
COMPARISON OF AN ENZYME-IMMUNOASSAY AND LATEX AGGLUTINATION-TEST FOR DETECTION OF GALACTOMANNAN IN THE DIAGNOSIS OF INVASIVE ASPERGILLOSIS
Autore:
SULAHIAN A; TABOURET M; RIBAUD P; SARFATI J; GLUCKMAN E; LATGE JP; DEROUIN F;
Indirizzi:
HOP ST LOUIS,LAB PARASITOL MYCOL,1 AVE CLAUDE VELLEFAUX F-75010 PARISFRANCE SANOFI DIAGNOST PASTEUR STEENVOORDE FRANCE HOP ST LOUIS,UNITE GREFFE MOELLE OSSEUSE F-75010 PARIS FRANCE INST PASTEUR,UNITE MYCOL MED PARIS FRANCE
Titolo Testata:
European journal of clinical microbiology & infectious diseases
fascicolo: 2, volume: 15, anno: 1996,
pagine: 139 - 145
SICI:
0934-9723(1996)15:2<139:COAEAL>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULMONARY ASPERGILLOSIS; ANTIGEN; RADIOIMMUNOASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
A. Sulahian et al., "COMPARISON OF AN ENZYME-IMMUNOASSAY AND LATEX AGGLUTINATION-TEST FOR DETECTION OF GALACTOMANNAN IN THE DIAGNOSIS OF INVASIVE ASPERGILLOSIS", European journal of clinical microbiology & infectious diseases, 15(2), 1996, pp. 139-145

Abstract

Aspergillus antigenemia was followed up in 215 consecutively observedbone marrow transplant (BMT) patients over a period of two years, using both a latex agglutination test and a sandwich immunocapture enzymeimmunoassay (EIA) with a rat anti-galactomannan monoclonal antibody as capture and detector antibody. For each patient, sequential sera (3 to 20) were obtained before and after BMT. No positivity was observed before BMT After BMT, the EIA and latex agglutination test were positive in 19 and 4 patients respectively of 25 patients with confirmed aspergillosis and 14 and 7 of 15 patients with probable aspergillosis. In19 of 25 patients with confirmed aspergillosis and 9 of 15 patients with probable aspergillosis, the EIA was more sensitive and detected infection earlier than the latex test. In all positive cases, antigenemia rapidly increased in sequential samples and remained strongly positive. In 31 of 169(19%) BMT patients without clinical signs of aspergillosis, the EIA was occasionally positive in samples taken within the first month after BMT, giving a specificity of 81% in these patients. Innon-BMT patients suffering from other diseases (n = 77), the specificity was 98.7%. The overall positive and negative predictive values forthe EIA were 54% and 95% respectively. These results favour the use of EIA for early diagnosis and monitoring of aspergillosis in BMT patients, although the predictive value of transient positivity remains to be ascertained.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 15:51:50